Microsatellite DNA marker development and genetic diversity ofBranchiostoma belcheriin Xiamen waters
| العنوان: | Microsatellite DNA marker development and genetic diversity ofBranchiostoma belcheriin Xiamen waters |
|---|---|
| المؤلفون: | Jing Zhong, Wei Xu, Yi-Quan Wang, Wei-Ye Li |
| المصدر: | Marine Biology Research. 7:826-831 |
| بيانات النشر: | Informa UK Limited, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Genetics, education.field_of_study, Genetic diversity, Linkage disequilibrium, animal structures, Library, Population, Population genetics, Aquatic Science, Biology, Oceanography, chemistry.chemical_compound, chemistry, Molecular marker, Microsatellite, Primer (molecular biology), education, Ecology, Evolution, Behavior and Systematics |
| الوصف: | Amphioxus occupies a transitional position between invertebrates and vertebrates and is therefore a promising laboratory model animal. As a powerful molecular marker in population genetics studies, microsatellite DNA is required for both the investigation of genetic diversity of a wild amphioxus population and strain breeding of laboratory cultured amphioxus. In the present study, we constructed a microsatellite-enriched DNA library of amphioxus Branchiostoma belcheri to develop this molecular marker. After screening the library and combining it with the data obtained from a public database, we successfully developed 21 pair of primers that could effectively amplify the microsatellite loci of amphioxus. Of those primer pairs, seven were in deviation of Hardy–Weinberg equilibrium, mostly caused by null alleles. The others had no linkage disequilibrium, indicating they are applicable for the genetic studies. Using those primer pairs, we further detected the genetic diversity of the wild amphioxus p... |
| تدمد: | 1745-1019 1745-1000 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::f2f10673259aa83b06e62e56dbc5377a https://doi.org/10.1080/17451000.2011.569553 |
| رقم الأكسشن: | edsair.doi...........f2f10673259aa83b06e62e56dbc5377a |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 17451019 17451000 |
|---|