P.122 Unbiased whole brain circuit interrogation reveals neurons restoring walking after spinal cord injury
| العنوان: | P.122 Unbiased whole brain circuit interrogation reveals neurons restoring walking after spinal cord injury |
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| المؤلفون: | N Cho, J Squair, N James, L Baud, A Leonhartsberger, K Sveistyte, K Galan, Q Barraud, M Goubran, L Batti, S Pages, M Gautier, T Hutson, C Kathe, A Bichat, O Rizzo, M Hodara, J Bloch, G Courtine |
| المصدر: | Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques. 49:S40-S41 |
| بيانات النشر: | Cambridge University Press (CUP), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Neurology, Neurology (clinical), General Medicine |
| الوصف: | Background: There is presently no cure for locomotor deficits after spinal cord injury (SCI). Very few therapies effectively target the brain due to poor understanding of the brain’s role post-SCI. Newly developed tissue clearing techniques have permitted unbiased three-dimensional circuit analysis, opening new opportunities for SCI-related brain interrogation. Methods: We established a novel brain interrogation pipeline by optimizing mouse brain clearing, imaging, and atlas registration. We leveraged a spontaneous recovery lateral hemisection model to analyze whole brain cell activity and connectivity with the lumbar cord using cFos immunolabelling and virus-mediated projection tracing. We identified a functionally and anatomically dynamic region correlating with recovery and interrogated its locomotor role with optogenetics. We assessed deep brain electrical stimulation (DBS) of this region in a more clinically relevant rat contusion SCI using an established bipedal robotic interface. Results: We unexpectedly uncovered the lateral hypothalamus (LH) to functionally and anatomically correlate with recovery. LHVglut2 optogenetic stimulation significantly augmented locomotor function. LH DBS in rats acutely robustly augmented bipedal locomotion post-SCI. Conclusions: This is the first demonstration of the LH’s role in locomotion post-SCI and is a novel DBS target that robustly augmented locomotor function, dependent on LH glutamatergic cells. LH DBS may be a promising intervention in humans. |
| تدمد: | 2057-0155 0317-1671 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::f71667ca6ff0d9903440aa82f0674152 https://doi.org/10.1017/cjn.2022.214 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........f71667ca6ff0d9903440aa82f0674152 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20570155 03171671 |
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