Use of NotI microarrays in analysis of epigenetic and structural changes in epithelial tumor genomes by the example of human chromosome 3
| العنوان: | Use of NotI microarrays in analysis of epigenetic and structural changes in epithelial tumor genomes by the example of human chromosome 3 |
|---|---|
| المؤلفون: | Olga Yu. Yurkevich, Vitaly I. Loginov, Surya Pavan Yenamandra, Lev L. Kisselev, E. R. Zabarovsky, Tatyana Ivanova, I. V. Pronina, Lev Petrenko, Veronika I. Zabarovska, Alexander V. Zelenin, E. R. Rakhmanaliev, T. V. Pavlova, Vladimir I. Kashuba, Olga V. Muravenko |
| المصدر: | Molecular Biology. 43:313-320 |
| بيانات النشر: | Pleiades Publishing Ltd, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Genetics, genomic DNA, Chromosome 3, Structural Biology, Bisulfite sequencing, Biophysics, Illumina Methylation Assay, Genomics, Epigenetics, Biology, DNA microarray, Genome, Molecular biology |
| الوصف: | A new comparative genome hybridization technology using NotI microarrays is described (Karolinska Institute International Patent WO02/086163). The method is based on comparative genome hybridization of NotI-enriched probes from tumor and normal genomic DNA with radically new NotI microarrays. A total of 181 NotI-binding loci of human chromosome 3 were assayed in 200 human malignant tissue samples from various organs: kidney, lung, breast, ovary, cervix, and prostate. The most significant portion (above 30%) of aberrations (deletions and methylation) were detected in NotI sites located in the MINT24, BHLHB2, RPL15, RARbeta1, ITGA9, RBSP3, VHL, and ZIC4 genes. This indicates that they may be associated with cancer development. Methylation of these genomic loci was confirmed by methylation-specific PCR and bisulfite sequencing. The results confirm that the proposed method can contribute to cancer genomics. |
| تدمد: | 1608-3245 0026-8933 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::f9f4b6bfe7e6b7b7d7a2f4f3c970d087 https://doi.org/10.1134/s0026893309020137 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........f9f4b6bfe7e6b7b7d7a2f4f3c970d087 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16083245 00268933 |
|---|