Abstract 11169: The HVCN1 Voltage-Gated Proton Channel Contributes to Ph Regulation in Canine Ventricular Myocytes
العنوان: | Abstract 11169: The HVCN1 Voltage-Gated Proton Channel Contributes to Ph Regulation in Canine Ventricular Myocytes |
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المؤلفون: | Jianyong Ma, Xiaoqian Gao, Yutian Li, Thomas DeCoursey, Gary E. Shull, Hong-sheng Wang |
المصدر: | Circulation. 144 |
بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2021. |
سنة النشر: | 2021 |
مصطلحات موضوعية: | Physiology (medical), Cardiology and Cardiovascular Medicine |
الوصف: | Regulation of intracellular pH (pH i ) in cardiomyocytes is crucial for cardiac function; however, currently known mechanisms for direct or indirect extrusion of acid from cardiomyocytes seem insufficient for energetically-efficient extrusion of the massive H + loads generated under in vivo conditions. In cardiomyocytes, voltage-sensitive H + channel activity mediated by the HVCN1 proton channel would be a highly efficient means of disposing of H + , while avoiding Na + -loading, as occurs during direct acid extrusion via Na + /H + exchange or indirect acid extrusion via Na + -HCO 3 - cotransport. PCR and immunoblotting demonstrated expression of HVCN1 mRNA and protein in canine heart. Patch clamp analysis of canine ventricular myocytes revealed a voltage-gated H + current that was highly H + -selective. The current was blocked by external Zn 2+ and the HVCN1 blocker 5-chloro-2-guanidinobenzimidazole (ClGBI). Both the gating and Zn 2+ blockade of the current were strongly influenced by the pH gradient across the membrane. All characteristics of the observed current were consistent with the known hallmarks of HVCN1-mediated H + current. Inhibition of HVCN1 and the NHE1 Na + /H + exchanger, singly and in combination, showed that either mechanism was largely sufficient to maintain pH i in beating cardiomyocytes, but that inhibition of both activities caused rapid acidification. These results show that HVCN1 is expressed in canine ventricular myocytes and provides a major H + -extrusion activity, with a capacity similar to that of NHE1. In the beating heart in vivo , this activity would allow Na + -independent extrusion of H + during each action potential and, when functionally coupled with anion transport mechanisms, could facilitate transport-mediated CO 2 disposal. |
تدمد: | 1524-4539 0009-7322 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::ff7a19dab302463907c81cdcf38a99a9 https://doi.org/10.1161/circ.144.suppl_1.11169 |
رقم الأكسشن: | edsair.doi...........ff7a19dab302463907c81cdcf38a99a9 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 15244539 00097322 |
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