Characterization of primary models of human trophoblast
| العنوان: | Characterization of primary models of human trophoblast |
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| المؤلفون: | Xiaohui Zhao, Ridma C. Fernando, Megan A. Sheridan, Lucy Gardner, Vicente Perez-Garcia, Margherita Y. Turco, Steven G.E. Marsh, Russell S. Hamilton, Qian Li, Ashley Moffett |
| المصدر: | Development (Cambridge, England) article-version (VoR) Version of Record Development |
| مصطلحات موضوعية: | Human Development, Trophoblast stem cells, Cell Culture Techniques, Primary cells, Human leukocyte antigen, Biology, Models, Biological, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Syncytiotrophoblast, Downregulation and upregulation, Models, HLA Antigens, Pregnancy, medicine, Organoid, Humans, Molecular Biology, reproductive and urinary physiology, Cells, Cultured, 030304 developmental biology, 0303 health sciences, Reproductive Biology, Cytotrophoblast, Stem Cells, Trophoblast, Trophoblast organoids, Cell Differentiation, Placentation, Cell biology, Trophoblasts, HLA, Organoids, MicroRNAs, medicine.anatomical_structure, embryonic structures, Female, Stem cell, 030217 neurology & neurosurgery, Developmental Biology |
| الوصف: | Two recently developed models, trophoblast organoids and trophoblast stem cells (TSCs), are useful tools to further the understanding of human placental development. Both differentiate from villous cytotrophoblast (VCT) to either extravillous trophoblast (EVT) or syncytiotrophoblast (SCT). Here, we compare the transcriptomes and miRNA profiles of these models to identify which trophoblast they resemble in vivo. Our findings indicate that TSCs do not readily undergo SCT differentiation and closely resemble cells at the base of the cell columns from where EVT derives. In contrast, organoids are similar to VCT and undergo spontaneous SCT differentiation. A defining feature of human trophoblast is that VCT and SCT are human leukocyte antigen (HLA) null, whereas EVT expresses HLA-C, -G and -E molecules. We find that trophoblast organoids retain these in vivo characteristics. In contrast, TSCs express classical HLA-A and HLA-B molecules, and maintain their expression after EVT differentiation, with upregulation of HLA-G. Furthermore, HLA expression in TSCs differs when grown in 3D rather than in 2D, suggesting that mechanical cues are important. Our results can be used to select the most suitable model for the study of trophoblast development, function and pathology. Summary: Characterization of trophoblast organoids and trophoblast stem cells as exciting models of human placentation enables the selection of the most suitable system to address specific research questions. |
| اللغة: | English |
| تدمد: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.199749 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::000398f1f60100248fe7feae8f04b915 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....000398f1f60100248fe7feae8f04b915 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779129 09501991 |
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| DOI: | 10.1242/dev.199749 |