Genome and metabolic engineering in non-conventional yeasts: Current advances and applications
| العنوان: | Genome and metabolic engineering in non-conventional yeasts: Current advances and applications |
|---|---|
| المؤلفون: | Ian Wheeldon, Ann-Kathrin Löbs, Cory Schwartz |
| المصدر: | Synthetic and Systems Biotechnology, Vol 2, Iss 3, Pp 198-207 (2017) Synthetic and Systems Biotechnology |
| بيانات النشر: | KeAi Communications Co., Ltd., 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Yarrowia lipolytica, Scheffersomyces stipitis, Commodity chemicals, DSB, double strand break, lcsh:Biotechnology, Biomedical Engineering, sgRNA, short (or single) guide RNA, Biology, Applied Microbiology and Biotechnology, Article, Pichia pastoris, Metabolic engineering, Hansenula polymorpha, 03 medical and health sciences, CRISPR, Clustered regularly interspaced short palindromic repeats, Kluyveromyces marxianus, Structural Biology, lcsh:TP248.13-248.65, Genetics, PAM, protospacer adjacent motif, Bioprocess, TALEN, transcription activator-like effector nucleases, lcsh:QH301-705.5, 2. Zero hunger, Kluyveromyces lactis, Yarrowia, biology.organism_classification, Yeast, 030104 developmental biology, Biochemistry, lcsh:Biology (General), NHEJ, nonhomologous end-joining, HR, homologous recombination, CRISPR-Cas9 |
| الوصف: | Microbial production of chemicals and proteins from biomass-derived and waste sugar streams is a rapidly growing area of research and development. While the model yeast Saccharomyces cerevisiae is an excellent host for the conversion of glucose to ethanol, production of other chemicals from alternative substrates often requires extensive strain engineering. To avoid complex and intensive engineering of S. cerevisiae, other yeasts are often selected as hosts for bioprocessing based on their natural capacity to produce a desired product: for example, the efficient production and secretion of proteins, lipids, and primary metabolites that have value as commodity chemicals. Even when using yeasts with beneficial native phenotypes, metabolic engineering to increase yield, titer, and production rate is essential. The non-conventional yeasts Kluyveromyces lactis, K. marxianus, Scheffersomyces stipitis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris have been developed as eukaryotic hosts because of their desirable phenotypes, including thermotolerance, assimilation of diverse carbon sources, and high protein secretion. However, advanced metabolic engineering in these yeasts has been limited. This review outlines the challenges of using non-conventional yeasts for strain and pathway engineering, and discusses the developed solutions to these problems and the resulting applications in industrial biotechnology. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::001d8badc0ff67ef1410a17f5effab88 http://www.sciencedirect.com/science/article/pii/S2405805X17300613 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....001d8badc0ff67ef1410a17f5effab88 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |