The determination of total and unbound midazolam in human plasma. A comparison of high performance liquid chromatography, gas chromatography and gas chromatography/mass spectrometry
| العنوان: | The determination of total and unbound midazolam in human plasma. A comparison of high performance liquid chromatography, gas chromatography and gas chromatography/mass spectrometry |
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| المؤلفون: | J. Rudi, J. X. de Vries, Ingeborg Walter-Sack, R. Conradi |
| المصدر: | Biomedical Chromatography. 4:28-33 |
| بيانات النشر: | Wiley, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Chromatography, Gas, genetic structures, Midazolam, Clinical Biochemistry, Ultrafiltration, Mass spectrometry, Biochemistry, High-performance liquid chromatography, Gas Chromatography-Mass Spectrometry, Analytical Chemistry, Drug Discovery, medicine, Humans, Molecular Biology, Chromatography, High Pressure Liquid, Pharmacology, Chromatography, Chemistry, Extraction (chemistry), Blood Proteins, General Medicine, Blood proteins, Gas chromatography, Gas chromatography–mass spectrometry, Protein Binding, medicine.drug |
| الوصف: | Midazolam concentrations in patients' plasma was determined after extraction with high performance liquid chromatography (HPLC), gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). GC was selected for routine plasma assays in terms of selectivity, simplicity, precision, accuracy and sensitivity (0.02 microgram/mL); HPLC analysis was less sensitive (0.1 microgram/mL) than GC; GC/MS was used for analysis validation. Plasma protein binding of midazolam was determined by GC in patients' plasma after in vitro incubation with midazolam, ultrafiltration and extraction; 5% of the drug was unbound to plasma proteins. Midazolam distribution in lipoprotein fractions separated by ultracentrifugation of plasma obtained from patients on prolonged midazolam treatment was also assayed by GC. |
| تدمد: | 1099-0801 0269-3879 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::001dca56a6e520e4f9c4ed9f6cbb7c6b https://doi.org/10.1002/bmc.1130040105 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....001dca56a6e520e4f9c4ed9f6cbb7c6b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990801 02693879 |
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