Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency
| العنوان: | Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency |
|---|---|
| المؤلفون: | Xiaohang Wang, Yunting Zhou, Yumin Zhang, Wei Xu, Junming Zhou, Yang Chen, Jun Tang, Zilin Sun, Bo Sun |
| المصدر: | Journal of Diabetes Research, Vol 2020 (2020) Journal of Diabetes Research |
| بيانات النشر: | Hindawi Limited, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Blood Glucose, Male, Vitamin, medicine.medical_specialty, Article Subject, Endocrinology, Diabetes and Metabolism, Blotting, Western, Biology, Permeability, Diseases of the endocrine glands. Clinical endocrinology, Proinflammatory cytokine, Islets of Langerhans, Mice, Random Allocation, chemistry.chemical_compound, Endocrinology, Insulin-Secreting Cells, Internal medicine, Insulin Secretion, medicine, Animals, Glucose homeostasis, Endocrine system, Intestinal Mucosa, Inflammation, geography, geography.geographical_feature_category, Intestinal permeability, Vitamin A Deficiency, Biodiversity, Organ Size, Glucose Tolerance Test, RC648-665, Islet, medicine.disease, Gastrointestinal Microbiome, Intestines, Molecular Typing, Disease Models, Animal, Liver, chemistry, Dysbiosis, Metabolic syndrome, Research Article, Hormone |
| الوصف: | Aims. The underlying mechanisms involved in Vitamin A- (VA-) related changes in glucose metabolic disorders remain unclear. Recent evidence suggests that intestinal microbiota is closely linked to the metabolic syndrome. Here, we explored whether and how intestinal microbiota affects glucose homeostasis in VA-deficient diet-fed mice. Methods. Six-week-old male C57BL/6 mice were randomly placed on either a VA-sufficient (VAS) or VA-deficient (VAD) diet for 10 weeks. Subsequently, a subclass of the VAD diet-fed mice was switched to a VA-deficient rescued (VADR) diet for an additional 8 weeks. The glucose metabolic phenotypes of the mice were assessed using glucose tolerance tests and immunohistochemistry staining. Changes in intestinal microbiota were assessed using 16S gene sequencing. The intestinal morphology, intestinal permeability, and inflammatory response activation signaling pathway were assessed using histological staining, western blots, quantitative-PCR, and enzyme-linked immunosorbent assays. Results. VAD diet-fed mice displayed reduction of tissue VA levels, increased area under the curve (AUC) of glucose challenge, reduced glucose-stimulated insulin secretion, and loss of β cell mass. Redundancy analysis showed intestinal microbiota diversity was significantly associated with AUC of glucose challenge and β cell mass. VAD diet-driven changes in intestinal microbiota followed the inflammatory response with increased intestinal permeability and higher mRNA expression of intestinal inflammatory cytokines through nuclear factor-κB signaling pathway activation. Reintroduction of dietary VA to VAD diet-fed mice restored tissue VA levels, endocrine hormone profiles, and inflammatory response, which are similar to those observed following VAS-controlled changes in intestinal microbiota. Conclusions. We found intestinal microbiota effect islet function via controlling intestinal inflammatory phenotype in VAD diet-fed mice. Intestinal microbiota influences could be considered as an additional mechanism for the effect of endocrine function in a VAD diet-driven mouse model. |
| وصف الملف: | text/xhtml |
| تدمد: | 2314-6753 2314-6745 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00671c6ff3d15b02b3f80c748df128be https://doi.org/10.1155/2020/2354108 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....00671c6ff3d15b02b3f80c748df128be |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23146753 23146745 |
|---|