A two-step bioconversion process for canolol production from rapeseed meal combining an Aspergillus niger feruloyl esterase and the fungus Neolentinus lepideus
| العنوان: | A two-step bioconversion process for canolol production from rapeseed meal combining an Aspergillus niger feruloyl esterase and the fungus Neolentinus lepideus |
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| المؤلفون: | Alexandra Bisotto, David Navarro, Christian Ginies, Frédéric Fine, Craig B. Faulds, Jean-Claude Sigoillot, Oscar Laguna, Jérôme Lecomte, Anne Lomascolo, Corinne Peyronnet, Elise Odinot |
| المساهمون: | Biodiversité et Biotechnologie Fongiques (BBF), École Centrale de Marseille (ECM)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Terres Inovia, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Terres Univia, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-École Centrale de Marseille (ECM), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Technical Centre for Oilseed Crops, Grain Legumes and Industrial Hemp (TERRES INOVIA, Pessac, France), Inter-Branch Organization for Vegetable Oils and Proteins (TERRES UNIVIA, Paris, France)., Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Lomascolo, Anne |
| المصدر: | Microorganisms Microorganisms, MDPI, 2017, 5 (4), ⟨10.3390/microorganisms5040067⟩ Microorganisms, MDPI, 2017, 5 (4), 17 p. ⟨10.3390/microorganisms5040067⟩ Microorganisms, 2017, 5 (4), 17 p. ⟨10.3390/microorganisms5040067⟩ Microorganisms; Volume 5; Issue 4; Pages: 67 Microorganisms, Vol 5, Iss 4, p 67 (2017) Microorganisms 67 (5), 17 p.. (2017) |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Microbiology (medical), canolol, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, bioconversion, composé volatil, Bioconversion, composé phénolique, chromatographie en phase gazeuse, chimie verte, Raw material, 01 natural sciences, 7. Clean energy, Microbiology, Aspergillus niger, feruloyl esterase, Neolentinus lepideus, rapeseed meal, sinapic acid, Article, chromatographie en phase liquide, 03 medical and health sciences, Hydrolysis, chemistry.chemical_compound, Q02 - Traitement et conservation des produits alimentaires, Feruloyl esterase, 010608 biotechnology, Virology, Sinapine, tourteau de colza, Food science, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS, Q60 - Traitement des produits agricoles non alimentaires, biology, Canolol, activité enzymatique, Q70 - Traitement des déchets agricoles, biology.organism_classification, 030104 developmental biology, lcsh:Biology (General), Biochemistry, chemistry |
| الوصف: | International audience; Rapeseed meal is a cheap and abundant raw material, particularly rich in phenolic compounds of biotechnological interest. In this study, we developed a two-step bioconversion process of naturally occurring sinapic acid (4-hydroxy-3,5-dimethoxycinnamic acid) from rapeseed meal into canolol by combining the complementary potentialities of two filamentous fungi, the micromycete Aspergillus niger and the basidiomycete Neolentinus lepideus. Canolol could display numerous industrial applications because of its high antioxidant, antimutagenic and anticarcinogenic properties. In the first step of the process, the use of the enzyme feruloyl esterase type-A (named AnFaeA) produced with the recombinant strain A. niger BRFM451 made it possible to release free sinapic acid from the raw meal by hydrolysing the conjugated forms of sinapic acid in the meal (mainly sinapine and glucopyranosyl sinapate). An amount of 39 nkat AnFaeA per gram of raw meal, at 55 °C and pH 5, led to the recovery of 6.6 to 7.4 mg of free sinapic acid per gram raw meal, which corresponded to a global hydrolysis yield of 68 to 76% and a 100% hydrolysis of sinapine. Then, the XAD2 adsorbent (a styrene and divinylbenzene copolymer resin), used at pH 4, enabled the efficient recovery of the released sinapic acid, and its concentration after elution with ethanol. In the second step, 3-day-old submerged cultures of the strain N.lepideus BRFM15 were supplied with the recovered sinapic acid as the substrate of bioconversion into canolol by a non-oxidative decarboxylation pathway. Canolol production reached 1.3 g/L with a molar yield of bioconversion of 80% and a productivity of 100 mg/L day. The same XAD2 resin, when used at pH 7, allowed the recovery and purification of canolol from the culture broth of N. lepideus. The two-step process used mild conditions compatible with green chemistry. |
| وصف الملف: | text; application/pdf |
| اللغة: | English |
| تدمد: | 2076-2607 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00b48f12e64380ab1fda379b9f609289 http://agritrop.cirad.fr/585642/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....00b48f12e64380ab1fda379b9f609289 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20762607 |
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