Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice
| العنوان: | Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice |
|---|---|
| المؤلفون: | Fang-Cheng Bi, Nan Yao, Zhen-Yi Chang, Ling-Yan Wang, Zhe Liu, Jian Li, Liqun Huang, Quan-Fang Zhang |
| المصدر: | Frontiers in Plant Science, Vol 11 (2020) Frontiers in Plant Science |
| بيانات النشر: | Frontiers Media S.A., 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Programmed cell death, Ceramide, caspase, chemistry.chemical_element, Plant Science, Calcium, lcsh:Plant culture, 01 natural sciences, Calcium in biology, 03 medical and health sciences, chemistry.chemical_compound, otorhinolaryngologic diseases, lcsh:SB1-1110, ceramide, programmed cell death, Caspase, Original Research, calcium, biology, Calcium channel, rice, food and beverages, Sphingolipid, Cell biology, 030104 developmental biology, chemistry, DIDS, biology.protein, 010606 plant biology & botany |
| الوصف: | Ceramide sphingolipids are major components of membranes. C2 and C6 ceramides induce programmed cell death (PCD) in animals and plants, and we previously showed that C2 and C6 ceramides induce PCD in rice (Oryza sativa) protoplasts. However, the mechanistic link between sphingolipids and PCD in rice remains unclear. Here, we observed that calcium levels increased rapidly after ceramide treatment. Moreover, the calcium channel inhibitor LaCl3 and the intracellular calcium chelator acetoxymethyl-1, 2-bis (2-aminophenoxy) ethic acid (BAPTA-AM) inhibited this calcium increase and prevented ceramide-induced PCD. Moreover, caspase-3-like protease activity increased significantly in C6 ceramide-treated protoplasts, and a caspase-specific inhibitor prevented C6 ceramide-induced cell death. We also detected the other typical PCD events including ATP loss. DIDS (4, 49-diisothiocyanatostilbene- 2, 29-disulfonic acid), an inhibitor of voltage-dependent anion channels (VDACs), decreased C6 ceramide-induced cell death. Together, this evidence suggests that mitochondria played an important role in C6 ceramide-induced PCD. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0135d6d7daa98b853bc82a6d71fc3763 https://www.frontiersin.org/article/10.3389/fpls.2020.00145/full |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0135d6d7daa98b853bc82a6d71fc3763 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |