Field validation of recombinant antigen immunoassays for diagnosis of Lassa fever
| العنوان: | Field validation of recombinant antigen immunoassays for diagnosis of Lassa fever |
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| المؤلفون: | Mambu Momoh, Megan L. Heinrich, Matthew L. Boisen, S. Humarr Khan, Peter C. Kulakosky, Mbalu Fonnie, Luis M. Branco, Michael Gbakie, Katherine J. Siddle, Duane J. Bush, Aaron E. Lin, Megan M. Rowland, Sarah M. Winnicki, Mohamed Fullah, Anna E. Lachenauer, Mahan Nekoui, Christian T. Happi, Pardis C. Sabeti, Kristian G. Andersen, Robert W. Cross, Robert F. Garry, Lansana Kanneh, John S. Schieffelin, Don Grant, Kayla G. Barnes, Jeffrey G. Shaffer, Erica Ollmann Saphire, Kathryn M. Hastie, Russell B. Wilson, Veronica J. Koroma, Diana K. S. Nelson, Thomas W. Geisbert, Anatoliy P. Koval, Jessica N. Hartnett, Onikepe O. Folarin, Dylan Kotliar, Augustine Goba, John Demby Sandi |
| المصدر: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, medicine.drug_class, viruses, 030231 tropical medicine, lcsh:Medicine, Validation Studies as Topic, Antibodies, Viral, Monoclonal antibody, medicine.disease_cause, Article, Sierra Leone, Sierra leone, law.invention, 03 medical and health sciences, Lassa Fever, 0302 clinical medicine, Antigen, law, Humans, Medicine, lcsh:Science, Lassa virus, Lassa fever, Antigens, Viral, Immunoassay, Rapid diagnostic test, Multidisciplinary, biology, business.industry, lcsh:R, virus diseases, medicine.disease, Virology, Recombinant Proteins, 3. Good health, Africa, Western, 030104 developmental biology, Immunoglobulin M, Recombinant DNA, biology.protein, lcsh:Q, Antibody, business |
| الوصف: | Lassa fever, a hemorrhagic fever caused by Lassa virus (LASV), is endemic in West Africa. It is difficult to distinguish febrile illnesses that are common in West Africa from Lassa fever based solely on a patient’s clinical presentation. The field performance of recombinant antigen-based Lassa fever immunoassays was compared to that of quantitative polymerase chain assays (qPCRs) using samples from subjects meeting the case definition of Lassa fever presenting to Kenema Government Hospital in Sierra Leone. The recombinant Lassa virus (ReLASV) enzyme-linked immunosorbant assay (ELISA) for detection of viral antigen in blood performed with 95% sensitivity and 97% specificity using a diagnostic standard that combined results of the immunoassays and qPCR. The ReLASV rapid diagnostic test (RDT), a lateral flow immunoassay based on paired monoclonal antibodies to the Josiah strain of LASV (lineage IV), performed with 90% sensitivity and 100% specificity. ReLASV immunoassays performed better than the most robust qPCR currently available, which had 82% sensitivity and 95% specificity. The performance characteristics of recombinant antigen-based Lassa virus immunoassays indicate that they can aid in the diagnosis of LASV Infection and inform the clinical management of Lassa fever patients. |
| تدمد: | 2045-2322 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0139d84def53f456a36dc8f1e18ff59b https://doi.org/10.1038/s41598-018-24246-w |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0139d84def53f456a36dc8f1e18ff59b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20452322 |
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