4-Methylumbelliferone inhibits enhanced hyaluronan synthesis and cell migration in pancreatic cancer cells in response to tumor-stromal interactions
| العنوان: | 4-Methylumbelliferone inhibits enhanced hyaluronan synthesis and cell migration in pancreatic cancer cells in response to tumor-stromal interactions |
|---|---|
| المؤلفون: | Keiji Hirata, Xiao‑Bo Cheng, Atsuhiro Koga, Shiro Kohi, Norihiro Sato |
| المصدر: | Oncology Letters |
| بيانات النشر: | Spandidos Publications, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, hyaluronan synthase, Cancer Research, Stromal cell, endocrine system diseases, Cell, pancreatic ductal adenocarcinoma, migration, 4-methylumbelliferone, hyaluronan, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Hyaluronic acid, medicine, HAS1, Migration Assay, biology, Cell migration, Articles, Cell cycle, Hyaluronan synthase, 030104 developmental biology, medicine.anatomical_structure, Oncology, chemistry, 030220 oncology & carcinogenesis, biology.protein, Cancer research |
| الوصف: | Hyaluronic acid (HA) in tumor stroma promotes tumor invasion and progression. 4-Methylumbelliferone (4-MU) is a potent HA synthesis inhibitor. In the present study, the effects of 4-MU on enhanced HA synthesis and cell migration in pancreatic ductal adenocarcinoma (PDAC) cells, in response to co-culture with stromal fibroblasts, was investigated. The HA concentration was determined using ELISA and a Transwell migration assay was used to analyze cell migratory capability. The mRNA expression levels of hyaluronan synthases (HAS1, HAS2 and HAS3) were determined using the quantitative polymerase chain reaction. Co-culture between Panc-1 cells and stromal fibroblasts markedly increased cell migration in association with increasing HA production, which was markedly associated with an increase in HAS3 mRNA expression. Treatment with 4-MU markedly decreased the HA production and cell migration of Panc-1 cells in the co-culture system. The results of the present study suggested that interactions between PDAC cells and stromal fibroblasts increased HA production, resulting in a marked increase in migration of PDAC cells, and 4-MU may be used as a chemotherapeutic agent to inhibit the enhanced migration of PDAC cells in response to tumor-stromal interactions. |
| تدمد: | 1792-1082 1792-1074 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::013f451a7271e565348d45720e942dee https://doi.org/10.3892/ol.2018.8147 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....013f451a7271e565348d45720e942dee |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
|---|