CD44-Associated Tn Antigen as a New Biomarker of Tumor Cells with Aberrant Glycosylation

التفاصيل البيبلوغرافية
العنوان: CD44-Associated Tn Antigen as a New Biomarker of Tumor Cells with Aberrant Glycosylation
المؤلفون: Alexander Filatov, Pichugin Av, N. V. Bovin, Arthur T. Kopylov, M L Shuvalova, Dmitriy Mazurov
المصدر: Biochemistry (Moscow). 85:1064-1071
بيانات النشر: Pleiades Publishing Ltd, 2020.
سنة النشر: 2020
مصطلحات موضوعية: Glycosylation, Lung Neoplasms, medicine.drug_class, Tn antigen, Biophysics, Adenocarcinoma of Lung, Monoclonal antibody, Biochemistry, Biochemistry, Genetics and Molecular Biology (miscellaneous), 03 medical and health sciences, Antigen, Biomarkers, Tumor, medicine, Humans, Antigens, Tumor-Associated, Carbohydrate, MUC1, 0303 health sciences, biology, Chemistry, 030302 biochemistry & molecular biology, Mucin, CD44, Antibodies, Monoclonal, General Medicine, Molecular biology, Hyaluronan Receptors, A549 Cells, Cancer cell, biology.protein, CRISPR-Cas Systems, Geriatrics and Gerontology, Antibody, Molecular Chaperones
الوصف: Tn antigen is a tumor-associated antigen that appears on cancer cells as a result of aberrant O-glycosylation. The most studied form of Tn antigen is found in mucins, in particular, in mucin 1 (MUC1). Antibodies against this form of Tn antigen are used to diagnose tumors, as well as to generate T-killers with a chimeric receptor. Some carcinomas do not carry MUC1 and antibodies of a different specificity are required to detect Tn antigen on these cells. In our work, we searched for anti-Tn antibodies without preliminary assumptions about the proteins that may be carriers of the Tn antigen. For this purpose, we obtained several pairs of isogenic cell lines with the wild type and knockout of the Cosmc gene, which is essential for correct protein O-glycosylation. Using the created lines as immunogens, we generated a monoclonal antibody AKC3, which reacted with the Cosmc-deficient A549 lung adenocarcinoma cells and did not bind to the wild-type cells. Using mass spectrometry, as well as co-immunoprecipitation, it was shown that the AKC3 antibody recognized the Tn antigen in the context of CD44 protein - a protein important for tumor growth. The AKC3 antibody can be used for tumor diagnosis, and to generate T cells with a chimeric receptor for treatment of tumors that do not express mucins.
تدمد: 1608-3040
0006-2979
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0163c66b3fe13752e8ab84b5a1d325cf
https://doi.org/10.1134/s0006297920090060
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....0163c66b3fe13752e8ab84b5a1d325cf
قاعدة البيانات: OpenAIRE