B-cell surface antigen B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2
| العنوان: | B-cell surface antigen B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2 |
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| المؤلفون: | Arnold S. Freedman, Chikao Morimoto, John G. Gribben, Lee M. Nadler, Claude D. Gimmi, K Sugita, Gordon J. Freeman |
| المصدر: | Proceedings of the National Academy of Sciences. 88:6575-6579 |
| بيانات النشر: | Proceedings of the National Academy of Sciences, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Antigens, Differentiation, T-Lymphocyte, CD3 Complex, T-Lymphocytes, T cell, Receptors, Antigen, T-Cell, Cell Communication, In Vitro Techniques, Biology, Lymphocyte Activation, Transfection, Cell Line, Interleukin 21, CD28 Antigens, Antigen, Antigens, CD, T-Lymphocyte Subsets, medicine, Animals, Humans, Cytotoxic T cell, Antigen-presenting cell, Interleukin 3, B-Lymphocytes, Multidisciplinary, Lymphokine, Antibodies, Monoclonal, Molecular biology, Antigens, Differentiation, B-Lymphocyte, medicine.anatomical_structure, Interleukin 12, Interleukin-2, Tetradecanoylphorbol Acetate, Interleukin-4, Research Article |
| الوصف: | Occupancy of the T-cell receptor complex does not appear to be a sufficient stimulus to induce a T-cell-mediated immune response. Increasing evidence suggests that cognate cell-cell interaction between an activated T cell and an antigen-presenting cell may provide such a stimulus. A candidate T-cell surface molecule for this costimulatory signal is the T-cell-restricted CD28 antigen. Following crosslinking with anti-CD28 mAb, suboptimally stimulated CD28+ T cells show increased proliferation and markedly increased secretion of a subset of lymphokines. Recently, the B-cell surface activation antigen B7 was shown to be a natural ligand for the CD28 molecule, and both B7 and CD28 are members of the immunoglobulin superfamily. Here we report that B7-transfected CHO cells can induce suboptimally activated CD28+ T cells to proliferate and secrete high levels of interleukin 2. The response is identical whether T cells are submitogenically stimulated with either phorbol myristate acetate or anti-CD3 to activate the T cells. This response is specific and can be totally abrogated with anti-B7 monoclonal antibody. As has previously been observed for anti-CD28 monoclonal antibody, B7 ligation induced secretion of interleukin 2 but not interleukin 4. We have previously demonstrated that B7 expression is restricted to activated B lymphocytes and interferon gamma-activated monocytes. Since these two cellular populations are involved in antigen presentation as well as cognate interaction with T lymphocytes, B7 is likely to represent a central constimulatory signal that is capable of amplifying an immune response. |
| تدمد: | 1091-6490 0027-8424 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0171c600bc187f2a34aac84a64306af8 https://doi.org/10.1073/pnas.88.15.6575 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0171c600bc187f2a34aac84a64306af8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10916490 00278424 |
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