Effective expansion of forkhead box P3⁺ regulatory T cells via early secreted antigenic target 6 and antigen 85 complex B from Mycobacterium tuberculosis
التفاصيل البيبلوغرافية
العنوان:
Effective expansion of forkhead box P3⁺ regulatory T cells via early secreted antigenic target 6 and antigen 85 complex B from Mycobacterium tuberculosis
The expansion of CD4 + CD25 + forkhead box (FOX)P3 + regulatory T (Treg) cells has been observed in patients with Mycobacterium (M.) tuberculosis; however, the mechanism of expansion remains to be elucidated. The aim of the present study was to examine the role of the early secreted antigenic target 6(ESAT-6) and antigen 85 complex B (Ag85B) from M. tuberculosis on Treg cell expansion. To investigate the sensitivity of peripheral blood cultures to the M. tuberculosis ESAT-6 and Ag85B antigens, the proportion of circulating CD4 + CD25 + FOXP3 + Treg cells was determined using flow cytometry and the levels of FOXP3 mRNA were determined using reverse transcription quantitative polymerase chain reaction. The mRNA levels of FOXP3 and the proportion of circulating CD4 + CD25 + FOXP3 + Treg cells were increased in multiplicitous drug-resistant tuberculosis patients compared with those in healthy controls and patients with latent tuber- culosis (TB) infection (LTBI) (P