Circulating miRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains
| العنوان: | Circulating miRNAs in Small Extracellular Vesicles Secreted by a Human Melanoma Xenograft in Mouse Brains |
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| المؤلفون: | Carlo Leonetti, Germana Falcone, Igea D'Agnano, Valentina Palmieri, Nicolò Panini, Carla Musa, Ingrid Cifola, Laura Vilardo, Ferdinando Scavizzi, Armando Felsani, Fabrizio Bonaventura, Ilaria Iannetti, Marta Nardella, Giulia Bolasco, Marcello Raspa, Beatrice Cardinali, Chiara Di Pietro, Loredana Guglielmi, Manuela Porru, Massimiliano Papi |
| المصدر: | Cancers Cancers, Vol 12, Iss 1635, p 1635 (2020) Volume 12 Issue 6 |
| بيانات النشر: | MDPI, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Circulating mirnas, Cancer Research, Melanoma, Biology, small extracellular vesicles, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, lcsh:RC254-282, Extracellular vesicles, Article, Oncology, Downregulation and upregulation, Tumor progression, microRNA, medicine, Cancer research, melanoma, circulating miRNAs, Human melanoma, Brain metastasis |
| الوصف: | The identification of liquid biomarkers remains a major challenge to improve the diagnosis of melanoma patients with brain metastases. Circulating miRNAs packaged into tumor-secreted small extracellular vesicles (sEVs) contribute to tumor progression. To investigate the release of tumor-secreted miRNAs by brain metastasis, we developed a xenograft model where human metastatic melanoma cells were injected intracranially in nude mice. The comprehensive profiles of both free miRNAs and those packaged in sEVs secreted by the melanoma cells in the plasma demonstrated that most (80%) of the sEV-associated miRNAs were also present in serum EVs from a cohort of metastatic melanomas, included in a publicly available dataset. Remarkably, among them, we found three miRNAs (miR-224-5p, miR-130a-3p and miR-21-5p) in sEVs showing a trend of upregulation during melanoma progression. Our model is proven to be valuable for identifying miRNAs in EVs that are unequivocally secreted by melanoma cells in the brain and could be associated to disease progression. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2072-6694 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0238d71eabc40228f231629865ff7e59 http://europepmc.org/articles/PMC7352810 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0238d71eabc40228f231629865ff7e59 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20726694 |
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