Human FAM154A (SAXO1) is a microtubule-stabilizing protein specific to cilia and related structures
| العنوان: | Human FAM154A (SAXO1) is a microtubule-stabilizing protein specific to cilia and related structures |
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| المؤلفون: | Derrick R. Robinson, Nicolas Landrein, Christophe Bosc, Lucie Chansel, Aline Papaxanthos-Roche, Mélanie Bonhivers, Thomas Trian, Annie Andrieux, Roger Marthan, Emmanuel Roche, Denis Dacheux, Benoit Roger |
| المساهمون: | Laboratoire d'études en Géophysique et océanographie spatiales (LEGOS), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2015, 128 (7), pp.1294-1307. ⟨10.1242/jcs.155143⟩ |
| بيانات النشر: | HAL CCSD, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Axoneme, Centriole, [SDV]Life Sciences [q-bio], [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Flagellum, Biology, Microtubules, 03 medical and health sciences, 0302 clinical medicine, Microtubule, Humans, Basal body, Cilia, Eye Proteins, ComputingMilieux_MISCELLANEOUS, Centrioles, 030304 developmental biology, 0303 health sciences, Undulipodium, Cilium, Microtubule organizing center, Cell Biology, Cell biology, Microtubule-Associated Proteins, 030217 neurology & neurosurgery |
| الوصف: | Cilia and flagella are microtubule-based organelles present at the surface of most cells, ranging from protozoa to vertebrates, in which these structures are implicated in processes from morphogenesis to cell motility. In vertebrate neurons, microtubule-associated MAP6 proteins stabilize cold-resistant microtubules through their Mn and Mc modules, and play a role in synaptic plasticity. Although centrioles, cilia and flagella have cold-stable microtubules, MAP6 proteins have not been identified in these organelles, suggesting that additional proteins support this role in these structures. Here, we characterize human FAM154A (hereafter referred to as hSAXO1) as the first human member of a widely conserved family of MAP6-related proteins specific to centrioles and cilium microtubules. Our data demonstrate that hSAXO1 binds specifically to centriole and cilium microtubules. We identify, in vivo and in vitro, hSAXO1 Mn modules as responsible for microtubule binding and stabilization as well as being necessary for ciliary localization. Finally, overexpression and knockdown studies show that hSAXO1 modulates axoneme length. Taken together, our findings suggest a fine regulation of hSAXO1 localization and important roles in cilium biogenesis and function. |
| اللغة: | English |
| تدمد: | 0021-9533 1477-9137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::026e8416cdb641aedb0360f133681fce https://hal.archives-ouvertes.fr/hal-02322739 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....026e8416cdb641aedb0360f133681fce |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219533 14779137 |
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