Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe
| العنوان: | Detection of the Bacillus anthracis gyrA Gene by Using a Minor Groove Binder Probe |
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| المؤلفون: | Bonnie M. Loveless, Rebecca Susan Kaplan, Elizabeth Bode, Melissa S. Frye, William Hurtle, Deanna Bridge, David A. Kulesh, Michelle House, David A. Norwood, Jeff Garrison |
| المصدر: | Journal of Clinical Microbiology. 42:179-185 |
| بيانات النشر: | American Society for Microbiology, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Genetic Markers, Microbiology (medical), Bacillus (shape), Base Sequence, biology, Sequence analysis, Molecular Sequence Data, Nucleic acid sequence, Bacillus cereus, Bacteriology, Sequence Analysis, DNA, biology.organism_classification, Sensitivity and Specificity, DNA gyrase, Molecular biology, Bacillus anthracis, Microbiology, DNA Gyrase, Genetic marker, Bacillus thuringiensis, bacteria, Chromatography, High Pressure Liquid |
| الوصف: | Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis , Bacillus cereus , and Bacillus thuringiensis . We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis . A real-time PCR assay was developed for the detection of B. anthracis . After analysis of the unique nucleotide sequence of the B. anthracis gyrA gene, a fluorescent 3′ minor groove binding probe was tested with 171 organisms from 29 genera of bacteria, including 102 Bacillus strains. The assay was found to be specific for all 43 strains of B. anthracis tested. In addition, a test panel of 105 samples was analyzed to evaluate the potential diagnostic capability of the assay. The assay showed 100% specificity, demonstrating the usefulness of the gyrA gene as a specific chromosomal marker for B. anthracis . |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::027207aa2da203a8281b91f4e3a63951 https://doi.org/10.1128/jcm.42.1.179-185.2004 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....027207aa2da203a8281b91f4e3a63951 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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