A novel simple technique for en face endothelial observations using water-soluble media – ‘thinned-wall’ preparations
| العنوان: | A novel simple technique for en face endothelial observations using water-soluble media – ‘thinned-wall’ preparations |
|---|---|
| المؤلفون: | L. Surchev, Lazar Jelev |
| المصدر: | Journal of Anatomy. 212:192-197 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Adult, Male, Histology, Materials science, Adolescent, Endothelium, Adventitia, Thin wall, Microscopy, Methods, medicine, Animals, Humans, Rats, Wistar, Child, Molecular Biology, Softening, Process (anatomy), Aorta, Ecology, Evolution, Behavior and Systematics, Aged, Histocytological Preparation Techniques, Cell Biology, Anatomy, Middle Aged, Rats, Water soluble, medicine.anatomical_structure, Child, Preschool, cardiovascular system, Female, Layer (electronics), Developmental Biology, Biomedical engineering |
| الوصف: | A new, easily applicable technique providing en face preparations for light microscopy observations of the rat aorta and human thin wall arteries is described here. The major steps of the technique include attachment of the fixed and flattened vessel with the endothelium face down on a glass slide, covered with a water-soluble adhesive medium; drying and softening the vessel wall with another water-soluble medium; removal of the adventitia and most of the media; detaching the layer by placing the glass slide in water; and final attachment of the layer with the endothelium upwards. On such 'thinned-wall' preparations, 40-50 microm in thickness, the stained endothelial cells are clearly visible. Because of the preparation thickness and the use of water-soluble media during the preparation, some subendothelial lipid accumulations, characteristic of the early stages of atherosclerosis process, are well preserved. |
| تدمد: | 1469-7580 0021-8782 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02a935e6c43ab1b93724cc7a4a508cff https://doi.org/10.1111/j.1469-7580.2007.00844.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....02a935e6c43ab1b93724cc7a4a508cff |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14697580 00218782 |
|---|