Local Surface Electric Field’s Effect on Adsorbed Proteins’ Orientation
| العنوان: | Local Surface Electric Field’s Effect on Adsorbed Proteins’ Orientation |
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| المؤلفون: | J.D. Sib, L. Chahed, K. Zellama, Ahmed Bouhekka, Yamina Brahmi, D. Benlakehal, A. Kebab, Nathalie Lemée, F. Kail, Larbi Filali, Y. Bouizem |
| المصدر: | Surfaces Volume 2 Issue 2 Pages 30-431 |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | nanocrystalline silicon, Nanostructure, Materials science, amorphous/crystalline silicon heterojunction, Nanocrystalline silicon, Infrared spectroscopy, 02 engineering and technology, biosensors, 010402 general chemistry, 021001 nanoscience & nanotechnology, Electrostatics, protein adsorption, 01 natural sciences, amide dipoles, 0104 chemical sciences, symbols.namesake, Adsorption, biomolecule orientation, Chemical physics, Electric field, symbols, 0210 nano-technology, Raman spectroscopy, Protein adsorption |
| الوصف: | Hydrogenated nanocrystalline silicon, while being non-charged and non-polar, could be an ideal candidate for the non-covalent and orientation-controlled immobilization of biomolecules thanks to local electric fields around nanocrystals. To that effect, the adsorption of bovine serum albumin on substrates with different densities of nanocrystals, revealed by Raman spectroscopy and X-ray diffraction, was studied using infrared spectroscopy and atomic force microscopy. It was found that the protein&ndash surface interactions followed different mechanisms depending on the nanostructure at the surface: hydrophobic on the non-crystalline part of the surface and electrostatic around the crystalline part. These electrostatic interactions were driven by the electric fields that arose at the junction between crystalline and amorphous structures. These electric fields were found to be strong enough to interact with the amide dipoles, thereby reorienting the adsorbed protein molecules on this part of the surface. Nevertheless, the adsorbed proteins were found to be denatured, which was due to the surface chemistry, and not affected by the nanostructure. |
| وصف الملف: | application/pdf |
| تدمد: | 2571-9637 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02addcb049bb3c8c4284f6ec50357fa7 https://doi.org/10.3390/surfaces2020030 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....02addcb049bb3c8c4284f6ec50357fa7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25719637 |
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