The cationic amino acid exporter Slc7a7 is induced and vital in zebrafish tissue macrophages with sustained efferocytic activity
| العنوان: | The cationic amino acid exporter Slc7a7 is induced and vital in zebrafish tissue macrophages with sustained efferocytic activity |
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| المؤلفون: | Mireille Carrère, Marion Le Bris, Philippe Herbomel, Ignaty Leshchiner, Chooyoung Baek, Doris Lou Demy, Ramil Noche, Wolfram Goessling, Muriel Tauzin |
| المساهمون: | Cellules Souches et Développement / Stem Cells and Development, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Broad Institute of MIT and Harvard (BROAD INSTITUTE), Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston], Harvard Medical School [Boston] (HMS), Macrophages et Développement de l’Immunité, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by grants to P.H. from the European Commission through the FP6 ‘ZF-Models’ Integrated Project, from the Fondation pour la Recherche Médicale (‘FRM 2012 team’ #DEQ20120323714, ‘FRM 2016 team’ #DEQ20160334881) and from the Agence Nationale de la Recherche Laboratoire d'Excellence Revive (Investissement d'Avenir, ANR-10-LABX-73), ANR-10-LABX-0073,REVIVE,Stem Cells in Regenerative Biology and Medicine(2010), European Project: 26837,ZF-MODELS |
| المصدر: | Journal of Cell Science Journal of Cell Science, Company of Biologists, 2020, 133 (20), pp.jcs249037. ⟨10.1242/jcs.249037⟩ Journal of Cell Science, 2020, 133 (20), pp.jcs249037. ⟨10.1242/jcs.249037⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Programmed cell death, [SDV]Life Sciences [q-bio], Population, microglia, Biology, 03 medical and health sciences, 0302 clinical medicine, medicine, Macrophage, Animals, Amino acid transporter, Amino Acids, education, Efferocytosis, Zebrafish, 030304 developmental biology, efferocytosis, 0303 health sciences, education.field_of_study, Microglia, Cell Biology, Zebrafish Proteins, biology.organism_classification, zebrafish, Cell biology, SLC7A7 Gene, macrophages, medicine.anatomical_structure, 030217 neurology & neurosurgery |
| الوصف: | International audience; Most tissues harbor a substantial population of resident macrophages. Here, we elucidate a functional link between the Slc7a7 cationic amino acid transporter and tissue macrophages. We identified a mutant zebrafish devoid of microglia due to a mutation in the slc7a7 gene. We found that in Slc7a7-deficient larvae, macrophages do enter the retina and brain to become microglia, but then die during the developmental wave of neuronal apoptosis, which triggers intense efferocytic work from them. A similar macrophage demise occurs in other tissues, at stages where macrophages have to engulf many cell corpses, whether due to developmental or experimentally triggered cell death. We found that Slc7a7 is the main cationic amino acid transporter expressed in macrophages of zebrafish larvae, and that its expression is induced in tissue macrophages within 1-2 h upon efferocytosis. Our data indicate that Slc7a7 is vital not only for microglia but also for any steadily efferocytic tissue macrophages, and that slc7a7 gene induction is one of the adaptive responses that allow them to cope with the catabolism of numerous dead cells without compromising their own viability. |
| اللغة: | English |
| تدمد: | 0021-9533 1477-9137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02c06405c136b56df96dd17937818c65 https://hal.archives-ouvertes.fr/hal-03040280/document |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....02c06405c136b56df96dd17937818c65 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219533 14779137 |
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