Accurate Serodetection of Asymptomatic Leishmania donovani Infection by Use of Defined Antigens
| العنوان: | Accurate Serodetection of Asymptomatic Leishmania donovani Infection by Use of Defined Antigens |
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| المؤلفون: | Yasuyuki Goto, Aarthy C. Vallur, Steven G. Reed, Dinesh Mondal, Raodoh Mohamath, Caroline Reinhart, Malcolm S. Duthie, Prakash Ghosh |
| المصدر: | Journal of Clinical Microbiology |
| بيانات النشر: | American Society for Microbiology, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), 030231 tropical medicine, Antibodies, Protozoan, Antigens, Protozoan, Asymptomatic, Sensitivity and Specificity, Serology, 03 medical and health sciences, 0302 clinical medicine, Blood serum, Direct agglutination test, parasitic diseases, Medicine, Humans, Serologic Tests, Asymptomatic Diseases, Bangladesh, business.industry, Leishmaniasis, Gold standard (test), medicine.disease, Virology, Recombinant Proteins, 030104 developmental biology, Visceral leishmaniasis, Immunology, Leishmaniasis, Visceral, Parasitology, medicine.symptom, business, Leishmania donovani |
| الوصف: | Infection with Leishmania donovani is typically asymptomatic, but a significant number of individuals may progress to visceral leishmaniasis (VL), a deadly disease that threatens 200 million people in areas where it is endemic. While diagnosis of acute VL has been simplified by the use of cost-effective confirmatory serological tests, similar standardized tools are not widely available for detecting asymptomatic infection, which can be 4 to 20 times more prevalent than active disease. A simple and accurate serological test that is capable of detecting asymptomatic L. donovani infection will be useful for surveillance programs targeting VL control and elimination. To address this unmet need, we evaluated recombinant antigens for their ability to detect serum antibodies in 104 asymptomatic L. donovani -infected individuals (qualified as positive for L. donovani -specific antibodies by direct agglutination test [DAT]) from the Mymensingh district of Bangladesh where VL is hyperendemic. The novel proteins rKR95 and rTR18 possessed the greatest potential and detected 69% of DAT-positive individuals, with rKR95 being more robust in reactivity. Agreement in results for individuals with high DAT responses, who are more likely to progress to VL disease, was 74%. When considered along with rK39, a gold standard antigen that is used to confirm clinical diagnosis of VL but that is now becoming widely used for surveillance, rKR95 and rTR18 conferred a sensitivity of 84% based on a theoretical combined estimate. Our data indicate that incorporating rKR95 and rTR18 with rK39 in serological tests amenable to rapid or high-throughput screening may enable simple and accurate detection of asymptomatic infection. Such tests will be important tools to measure L. donovani infection rates, a primary goal in surveillance and a critical measurement with which to assess elimination programs. |
| اللغة: | English |
| تدمد: | 1098-660X 0095-1137 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02ccf4066b682669e729fad6c5389d31 http://europepmc.org/articles/PMC4809943 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....02ccf4066b682669e729fad6c5389d31 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1098660X 00951137 |
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