Human melanoma TrkC: Its association with a purine-analog-sensitive kinase activity
| العنوان: | Human melanoma TrkC: Its association with a purine-analog-sensitive kinase activity |
|---|---|
| المؤلفون: | Brian P. Murry, Andrea Wilke-Greiter, Dario Marchetti, Jennifer Galjour |
| المصدر: | Journal of Cellular Biochemistry. 88:865-872 |
| بيانات النشر: | Wiley, 2003. |
| سنة النشر: | 2003 |
| مصطلحات موضوعية: | Time Factors, Receptors, Nerve Growth Factor, Protein Serine-Threonine Kinases, Tropomyosin receptor kinase A, Receptor, Nerve Growth Factor, Biochemistry, Tropomyosin receptor kinase C, Neurotrophin 3, Cell Line, Tumor, Humans, Low-affinity nerve growth factor receptor, Receptor, trkC, Neoplasm Metastasis, Kinase activity, 2-Aminopurine, Thioguanine, Receptor, Melanoma, Molecular Biology, Dose-Response Relationship, Drug, biology, Brain Neoplasms, Kinase, Cell Biology, Nerve growth factor, nervous system, biology.protein, Cancer research, Protein Kinases, Signal Transduction, Neurotrophin |
| الوصف: | ThevariousmembersoftheTrktyrosinekinasefamilyandp75neurotrophinreceptor(p75 NTR )havebeen identifiedassignalingreceptorsforthestructurallyrelatedmembersoftheneurotrophins(NT)family.Wehavepreviously reported that NT treatment of murine and human brain-metastatic melanoma cells affects their invasive capacities and increases the production of extracellular-matrix degradative enzymes. These cells express aberrant levels of functional p75 NTR and TrkC, the putative high-affinity receptor for the neurotrophin NT-3. Here we demonstrate that, by using sensitive immune-complex kinase assays in human brain-metastatic (70W) melanoma cells, TrkC receptors associate with a kinase activity exhibiting a dose-dependent susceptibility to inhibition by the purine-analogs 6-thioguanine and 2-aminopurine. The activity of this purine-analog-sensitive kinase (PASK) was induced by NT-3 in a time-dependent fashion,phosphorylatingexogenousmyelinbasicprotein(MBP)butnotdenaturedenolase.Itissimilartotheonereported to relate with p75 NTR and TrkA receptors and stimulated by the prototypic NT, nerve growth factor. Thus, PASKs may represent unique signaling components common to NT receptors that could engage joint downstream signaling effectors in brain-metastatic melanoma. J. Cell. Biochem. 88: 865-872, 2003. 2003 Wiley-Liss, Inc. |
| تدمد: | 1097-4644 0730-2312 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::032e70005ee4948acb0ae1319b30cb39 https://doi.org/10.1002/jcb.10473 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....032e70005ee4948acb0ae1319b30cb39 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 |
|---|