Determination of ambroxol in human plasma by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-MS/ESI)

التفاصيل البيبلوغرافية
العنوان: Determination of ambroxol in human plasma by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC-MS/ESI)
المؤلفون: Huan-De Li, Wei Gao, Fenli Su, Feng Wang
المصدر: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 853(1-2)
سنة النشر: 2007
مصطلحات موضوعية: Detection limit, Electrospray, Spectrometry, Mass, Electrospray Ionization, Chromatography, Molecular Structure, Chemistry, Formic acid, Electrospray ionization, Clinical Biochemistry, Analytical chemistry, Reproducibility of Results, Cell Biology, General Medicine, Mass spectrometry, Biochemistry, High-performance liquid chromatography, Analytical Chemistry, chemistry.chemical_compound, Ambroxol, Liquid chromatography–mass spectrometry, Humans, Selected ion monitoring, Chromatography, High Pressure Liquid
الوصف: A rapid, sensitive and specific method to determination of ambroxol in human plasma using high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC–MS/ESI) was described. Ambroxol and the internal standard (I.S.), fentanyl, were extracted from plasma by N-hexane-diethyl ether (1:1, v/v) after alkalinized with ammonia water. A centrifuged upper layer was then evaporated and reconstituted with 100 μl mobile phase. Chromatographic separation was performed on a BDS HYPERSIL C18 column (250 mm × 4.6 mm, 5.0 μm, Thermo electron corporation, USA) with the mobile phase consisting of 30 mM ammonium acetate (0.4% formic acid)–acetonitrile (64:36, v/v) at a flow-rate of 1.2 mL min−1. The total run time was 5.8 min for each sample. Detection and quantitation was performed by the mass spectrometer using selected ion monitoring at m/z 261.9, 263.8 and 265.9 for ambroxol and m/z 337.3 for fentanyl. The calibration curve was linear within the concentration range of 1.0–100.0 ng mL−1 (r = 0.9996). The limit of quantification was 1.0 ng mL−1. The extraction recovery was above 83.3%. The methodology recovery was higher than 93.8%. The intra- and inter-day precisions were less than 6.0%. The method is accurate, sensitive and simple for the study of the pharmacokinetics and metabolism of ambroxol.
تدمد: 1570-0232
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03301a137db3527c7d3c0fdced0d1f50
https://pubmed.ncbi.nlm.nih.gov/17446147
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....03301a137db3527c7d3c0fdced0d1f50
قاعدة البيانات: OpenAIRE