Transcriptional and Post-Transcriptional Control of Tubulin Gene Expression inTrypanosoma cruzi
| العنوان: | Transcriptional and Post-Transcriptional Control of Tubulin Gene Expression inTrypanosoma cruzi |
|---|---|
| المؤلفون: | Rondinelli E, Turán P. Ürményi, De Souza W, J. F. Carvalho, De Castro Ft |
| المصدر: | DNA and Cell Biology. 11:101-109 |
| بيانات النشر: | Mary Ann Liebert Inc, 1992. |
| سنة النشر: | 1992 |
| مصطلحات موضوعية: | Cell type, Transcription, Genetic, Trypanosoma cruzi, macromolecular substances, Vinblastine, chemistry.chemical_compound, Tubulin, Gene expression, Genetics, medicine, Animals, RNA Processing, Post-Transcriptional, Molecular Biology, Post-transcriptional regulation, Messenger RNA, biology, Cell Biology, General Medicine, Blotting, Northern, biology.organism_classification, Molecular biology, Kinetics, Microscopy, Electron, Gene Expression Regulation, chemistry, biology.protein, Growth inhibition, medicine.drug |
| الوصف: | We have shown that tubulin mRNA accumulation is regulated at the transcriptional level during metacyclogenesis of Trypanosoma cruzi, although the contribution of post-transcriptional mechanisms is also indicated. mRNA heterogeneity is not restricted to beta-tubulin, and differential regulation of alpha-tubulin mRNAs is observed during this stage of the parasite's life cycle. Treatment of epimastigotes with the microtubule-depolymerizing agent vinblastine resulted in growth inhibition and morphological alterations. Vinblastine also induced a rise in the pool of free tubulin subunits, concomitant with diminished tubulin synthesis and reduced mRNA levels. Tubulin gene transcription remained unaltered during vinblastine treatment, suggesting post-transcriptional control. These observations are in agreement with the autoregulatory model of tubulin gene expression described for a variety of cell types. We conclude that T. cruzi utilizes transcriptional and post-transcriptional control mechanisms for tubulin gene expression. |
| تدمد: | 1557-7430 1044-5498 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::033fe78388dedbc8041e1ce6ba3fd37f https://doi.org/10.1089/dna.1992.11.101 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....033fe78388dedbc8041e1ce6ba3fd37f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15577430 10445498 |
|---|