Straightforward, One-Step Fabrication of Ultrathin Thermoresponsive Films from Commercially Available pNIPAm for Cell Culture and Recovery
| العنوان: | Straightforward, One-Step Fabrication of Ultrathin Thermoresponsive Films from Commercially Available pNIPAm for Cell Culture and Recovery |
|---|---|
| المؤلفون: | Peter Dockery, Catherine Liptrot, Amaya Garcia, Natalia Nikoloskya, Fiona M. Lyng, Alexander V. Gorelov, Maria E. Nash, Rongbing Yang, Yury Rochev, Claire O' Connell, William M. Carroll |
| المصدر: | ACS Applied Materials & Interfaces. 3:1980-1990 |
| بيانات النشر: | American Chemical Society (ACS), 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Spin coating, Aqueous solution, Fabrication, Materials science, Polymers, Acrylic Resins, Temperature, One-Step, Nanotechnology, 3T3 Cells, Polymer, Lower critical solution temperature, Mice, chemistry.chemical_compound, chemistry, Cell culture, Cell Adhesion, Poly(N-isopropylacrylamide), Animals, General Materials Science |
| الوصف: | The use of thermoresponsive surfaces as platforms for cell culture and cell regeneration has been explored over the last couple of decades. Poly-N-isopropylacrylamide (pNIPAm) is a well characterized thermoresponsive polymer which has an aqueous lower critical solution temperature (LCST) in a physiologically useful range, which allows it to reversibly attract (T32 °C) and repel water (T32 °C). It is this phenomenon that is exploited in temperature-controlled cell harvesting. pNIPAm coatings are generally poorly cell compatible and a number of complex or expensive techniques have been developed in order to overcome this issue. This study seeks to design a simple one-step system whereby commercially sourced pNIPAm is used to achieve similar results. Films were deposited using the operationally simple but rheologically complex spin coating technique. Reversible temperature modulated cell adhesion was achieved using a variety of different cell lines. This system offers a simplistic and cheaper alternative to methods used elsewhere. |
| تدمد: | 1944-8252 1944-8244 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03586359d932881fda24d7656ffb9b57 https://doi.org/10.1021/am200204j |
| رقم الأكسشن: | edsair.doi.dedup.....03586359d932881fda24d7656ffb9b57 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 19448252 19448244 |
|---|