Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach
| العنوان: | Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach |
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| المؤلفون: | Peter van Ulsen, Thorsten Prinz, Peter Schmid, Harald Legner, Jan Tommassen, Karsten Kuhn, Christian Baumann |
| المساهمون: | Molecular Microbiology |
| المصدر: | Journal of Proteomics, 1771-1781. Elsevier ISSUE=7;STARTPAGE=1771;ENDPAGE=1781;ISSN=1874-3919;TITLE=Journal of Proteomics van Ulsen, J P, Kuhn, K, Prinz, T, Legner, H, Schmid, P, Baumann, C & Tommassen, J 2009, ' Identification of proteins of Neisseria meningitidis induced under iron-limiting conditions using the isobaric tandem mass tag (TMT) labeling approach. ', Journal of Proteomics, no. 7, pp. 1771-1781 . https://doi.org/10.1002/pmic.200800642 |
| بيانات النشر: | Elsevier, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Gene Expression Profiling, Iron, Neisseria meningitidis, Reproducibility of Results, Gene Expression Regulation, Bacterial, Genomics, Metabolism, Limiting, Biology, medicine.disease_cause, Tandem mass tag, Biochemistry, Isobaric labeling, Bacterial Proteins, Tandem Mass Spectrometry, medicine, Isobaric process, ORFS, DNA microarray, Molecular Biology, Oligonucleotide Array Sequence Analysis |
| الوصف: | Isobaric labeling reagents such as Tandem Mass Tags (TMT®) enable the genome-wide quantification of protein expression levels under different conditions using a gel-free MS/MS-based approach. Here, we applied a TMTduplex approach with two isobaric tags to study the response of the human pathogen Neisseria meningitidis to deprivation of iron, a condition met in the human body. In total, 609 proteins were identified in samples of three independent growth experiments, in which we compared cultures grown in the presence and absence of iron. Expression of 35 proteins was found to be induced or repressed under iron-limiting conditions, including 11 proteins whose ORFs were not previously identified in DNA array studies as being regulated by iron availability at the transcriptional level. These 11 proteins include proteins likely involved in iron metabolism. ©2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| اللغة: | English |
| تدمد: | 1876-7737 1874-3919 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03ad4142e105a6bfd850b043d28b7392 https://research.vu.nl/en/publications/e8ac30d8-2562-40c8-b37d-a35f26f46884 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....03ad4142e105a6bfd850b043d28b7392 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18767737 18743919 |
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