A portable smartphone-based imaging surface plasmon resonance biosensor for allergen detection in plant-based milks
| العنوان: | A portable smartphone-based imaging surface plasmon resonance biosensor for allergen detection in plant-based milks |
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| المؤلفون: | Chi Xiao, Georgina Ross, Michel W.F. Nielen, Jens Eriksson, Gert IJ. Salentijn, Wing Cheung Mak |
| المصدر: | Talanta, 257 Talanta 257 (2023) |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Immunoassay, Smartphone analysis, Plant-based milk, Programme and Account Management, Point of need, Organic Chemistry, Food allergens, Team Bioassays & Biosensors, Portable surface plasmon resonance, Organische Chemie, Analytical Chemistry, VLAG |
| الوصف: | Food allergies are hypersensitivity immune responses triggered by (traces of) allergenic compounds in foods and drinks. The recent trend towards plant-based and lactose-free diets has driven an increased consumption of plant-based milks (PBMs) with the risk of cross-contamination of various allergenic plant-based proteins during the food manufacturing process. Conventional allergen screening is usually performed in the laboratory, but portable biosensors for on-site screening of food allergens at the production site could improve quality control and food safety. Here, we developed a portable smartphone imaging surface plasmon resonance (iSPR) biosensor composed of a 3D-printed microfluidic SPR chip for the detection of total hazelnut protein (THP) in commercial PBMs and compared its instrumentation and analytical performance with a conventional benchtop SPR. The smartphone iSPR shows similar characteristic sensorgrams compared with the benchtop SPR and enables the detection of trace levels of THP in spiked PBMs with the lowest tested concentration of 0.625 μg/mL THP. The smartphone iSPR achieved LoDs of 0.53, 0.16, 0.14, 0.06, and 0.04 μg/mL THP in 10x-diluted soy, oat, rice, coconut, and almond PBMs, respectively, with good correlation with the conventional benchtop SPR system (R2 0.950–0.991). The portability and miniaturized characteristics of the smartphone iSPR biosensor platform make it promising for the future on-site detection of food allergens by food producers. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0039-9140 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03d5ad44dfe8ae70c7ad4461e3e5c9f7 https://research.wur.nl/en/publications/a-portable-smartphone-based-imaging-surface-plasmon-resonance-bio |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....03d5ad44dfe8ae70c7ad4461e3e5c9f7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00399140 |
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