Multilocus sequence typing of Mycoplasma agalactiae
| العنوان: | Multilocus sequence typing of Mycoplasma agalactiae |
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| المؤلفون: | M.L. de Garnica, F. Gosney, Richard J. Ellis, Laura McAuliffe, M. Kargl, Robin A.J. Nicholas, Miroslav Hlusek, Joachim Spergser, Roger D. Ayling, Renate Rosengarten |
| المصدر: | Journal of Medical Microbiology. 60:803-811 |
| بيانات النشر: | Microbiology Society, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | DNA, Bacterial, Microbiology (medical), Genotype, Mycoplasma agalactiae, ved/biology.organism_classification_rank.species, Sheep Diseases, Biology, Microbiology, Genome, Animals, Mycoplasma Infections, Typing, Sequence (medicine), Genetics, Molecular Epidemiology, Genetic diversity, Goat Diseases, Sheep, ved/biology, Goats, Strain (biology), General Medicine, Bacterial Typing Techniques, Genes, Bacterial, Spain, bacteria, Multilocus sequence typing, Genome, Bacterial, Multilocus Sequence Typing |
| الوصف: | Mycoplasma agalactiae is the main cause of contagious agalactia, a serious disease of sheep and goats, which has major clinical and economic impacts. We have developed a multilocus sequence typing (MLST) scheme using the sequenced genomes of the M. agalactiae strains PG2 and 5632. An MLST scheme based on the genes gltX, metS, gyrB, tufA and dnaA was designed and in total 3468 bp of sequence were analysed for each strain. MLST offers a highly discriminatory typing method for M. agalactiae and was capable of subdividing 53 strains into 17 distinct sequence types, largely according to geographical origin. MLST detected unexpected diversity in recent isolates from Spain, identifying two novel outliers, and enabled typing of novel Mongolian isolates for the first time. Genetic diversity in the sequenced regions was largely due to mutation, with recombination playing a much smaller role. A web-accessible database has been set up for this MLST scheme for M. agalactiae: http://pubmlst.org/magalactiae/. MLST offers a robust, objective molecular epidemiological tool for M. agalactiae that that enables interlaboratory comparison of data. |
| تدمد: | 1473-5644 0022-2615 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0440c74d19965fd720c4cc33c84e9830 https://doi.org/10.1099/jmm.0.028159-0 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0440c74d19965fd720c4cc33c84e9830 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14735644 00222615 |
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