Microscopic and biomolecular complementary approaches to characterize bioweathering processes at petroglyph sites from the Negev Desert, Israel
| العنوان: | Microscopic and biomolecular complementary approaches to characterize bioweathering processes at petroglyph sites from the Negev Desert, Israel |
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| المؤلفون: | Hana Barak, Asunción de los Ríos, Irit Nir, Ariel Kushmaro, Esti Kramarsky-Winter |
| المساهمون: | Comunidad de Madrid, European Commission |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| بيانات النشر: | Society for Applied Microbiology, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0303 health sciences, biology, Bacteria, 030306 microbiology, Ecology, Shotgun sequencing, Weathering, Substrate (biology), biology.organism_classification, Microbiology, Cultural heritage, 03 medical and health sciences, Metagenomics, Microscopy, Electron, Scanning, Petroglyph, Rock art, Israel, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, Archaea |
| الوصف: | Throughout the Negev Desert highlands, thousands of ancient petroglyphs sites are susceptible to deterioration processes that may result in the loss of this unique rock art. Therefore, the overarching goal of the current study was to characterize the composition, diversity and effects of microbial colonization of the rocks to find ways of protecting these unique treasures. The spatial organization of the microbial colonizers and their relationships with the lithic substrate were analysed using scanning electron microscopy. This approach revealed extensive epilithic and endolithic colonization and close microbial–mineral interactions. Shotgun sequencing analysis revealed various taxa from the archaea, bacteria and some eukaryotes. Metagenomic coding sequences (CDS) of these microbial lithobionts exhibited specific metabolic pathways involved in the rock elements' cycles and uptake processes. Thus, our results provide evidence for the potential participation of the microorganisms colonizing these rocks during different solubilization and mineralization processes. These damaging actions may contribute to the deterioration of this extraordinary rock art and thus threaten this valuable heritage. Shotgun metagenomic sequencing, in conjunction with the in situ scanning electron microscopy study, can thus be considered an effective strategy to understand the complexity of the weathering processes occurring at petroglyph sites and other cultural heritage assets. The microscopy study was supported by program TOP-HERITAGE S2018/NMT-4372 (Comunidad de Madrid, FSE, FEDER). |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04679d033f846ac19839b82429cffb5a http://hdl.handle.net/10261/249160 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....04679d033f846ac19839b82429cffb5a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |