Extrapancreatic effects of incretin hormones: evidence for weight-independent changes in morphological aspects and oxidative status in insulin-sensitive organs of the obese nondiabetic Zucker rat (ZFR)
| العنوان: | Extrapancreatic effects of incretin hormones: evidence for weight-independent changes in morphological aspects and oxidative status in insulin-sensitive organs of the obese nondiabetic Zucker rat (ZFR) |
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| المؤلفون: | Henri Colin, Ides M. Colin, Jean Saey, Anne-Catherine Gérard, Bernard Knoops, Inès Dufour, Charles‐Edouard Gielen, Marie-Christine Many |
| المساهمون: | UCL - SST/ISV - Institut des sciences de la vie |
| المصدر: | Physiological Reports Physiological Reports, Vol. 4, no.15, p. 1-13 (2016) |
| بيانات النشر: | Wiley, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | Blood Glucose, Male, 0301 basic medicine, Physiology, medicine.medical_treatment, Adipose tissue, Type 2 diabetes, medicine.disease_cause, Signalling Pathways, incretin hormones, Lipid droplet, exendin-4, Brown adipose tissue, oxidative stress, Insulin, Original Research, Organ Size, Zucker rat, medicine.anatomical_structure, Adipose Tissue, Organ Specificity, Endocrine and Metabolic Conditons, Disorders and Treatments, Adipose Tissue and Obesity, medicine.medical_specialty, Incretin, Biology, Incretins, 03 medical and health sciences, Physiology (medical), Internal medicine, Metabolism and Regulation, medicine, Animals, Muscle, Skeletal, Venoms, Body Weight, medicine.disease, Rats, Rats, Zucker, 030104 developmental biology, Endocrinology, inflammation, Hepatocytes, Exenatide, Exendin‐4, Insulin Resistance, Peptides, Oxidative stress, Hormone |
| الوصف: | Incretin‐based therapies are widely used to treat type 2 diabetes. Although hypoglycemic actions of incretins are mostly due to their insulinotropic/glucagonostatic effects, they may also influence extrapancreatic metabolism. We administered exendin‐4 (Ex‐4), a long‐acting glucagon‐like peptide receptor agonist, at low dose (0.1 nmol/kg/day) for a short period (10 days), in obese nondiabetic fa/fa Zucker rats (ZFRs). Ex‐4‐treated ZFRs were compared to vehicle (saline)‐treated ZFRs and vehicle‐ and Ex‐4‐treated lean rats (LRs). Blood glucose levels were measured at days 0, 9, and 10. Ingested food and animal weight were recorded daily. On the day of sacrifice (d10), blood was sampled along with liver, epididymal, subcutaneous, brown adipose, and skeletal muscle tissues from animals fasted for 24 h. Plasma insulin and blood glucose levels, food intake, and body and epididymal fat weight were unchanged, but gross morphological changes were observed in insulin‐sensitive tissues. The average size of hepatocytes was significantly lower in Ex‐4‐treated ZFRs, associated with decreased number and size of lipid droplets and 4‐hydroxy‐2‐nonenal (HNE) staining, a marker of oxidative stress (OS). Myocytes, which were smaller in ZFRs than in LRs, were significantly enlarged and depleted of lipid droplets in Ex‐4‐treated ZFRs. Weak HNE staining was increased by Ex‐4. A similar observation was made in brown adipose tissue, whereas the elevated HNE staining observed in epididymal adipocytes of ZFRs, suggestive of strong OS, was decreased by Ex‐4. These results suggest that incretins by acting on OS in insulin‐sensitive tissues may contribute to weight‐independent improvement in insulin sensitivity. |
| تدمد: | 2051-817X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::050f66768d0e5221f9419e5af5fcc64f https://doi.org/10.14814/phy2.12886 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....050f66768d0e5221f9419e5af5fcc64f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2051817X |
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