Algal fluorescence sensor integrated into a microfluidic chip for water pollutant detection
| العنوان: | Algal fluorescence sensor integrated into a microfluidic chip for water pollutant detection |
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| المؤلفون: | Luping Yu, Annie Chalifour, Philippe Juneau, Florent Lefèvre, Vamsy P. Chodavarapu, Ricardo Izquierdo |
| المصدر: | Lab Chip. 12:787-793 |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Materials science, Fabrication, Biomedical Engineering, Photodetector, Bioengineering, Biochemistry, Fluorescence, Algae, OLED, Dimethylpolysiloxanes, Chlorophyll fluorescence, Pollutant, biology, Herbicides, business.industry, General Chemistry, Microfluidic Analytical Techniques, biology.organism_classification, Nylons, Optoelectronics, Biological Assay, Green algae, business, Chlamydomonas reinhardtii, Water Pollutants, Chemical |
| الوصف: | We report the first miniaturized fluorescent sensor based on algae, with an organic light emitting diode (OLED) and an organic photodetector (OPD) integrated into a microfluidic chip. The blue emission OLED was used as the excitation source, while a blend of PTB3/PC(61)BM was used for the fabrication of the organic photodetector. Excitation and emission color filters based on acid/base dyes and a metal complex were developed and assembled with the organic optoelectronic components in order to complete the fluorescent detection system. The detection system was then integrated in a microfluidic chip made from (poly)dimethylsiloxane (PDMS). The complete sensor is designed to detect algal fluorescence in the microfluidic chamber. Algal chlorophyll fluorescence enables evaluation of the toxicity of pollutants like herbicides and metals-ions from agricultural run-offs. The entirely organic bioassay here presented allowed detection of the toxic effects of the herbicide Diuron on Chlamydomonas reinhardtii green algae that gave 50% inhibition of the algae photochemistry (EC(50)) with a concentration as low as 11 nM. |
| تدمد: | 1473-0189 1473-0197 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::052b2a18e33ccb8b8cbbe96c0fc4238e https://doi.org/10.1039/c2lc20998e |
| رقم الأكسشن: | edsair.doi.dedup.....052b2a18e33ccb8b8cbbe96c0fc4238e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14730189 14730197 |
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