Selection of lymphocyte gating protocol has an impact on the level of reliability of T-cell subsets in aging specimens
| العنوان: | Selection of lymphocyte gating protocol has an impact on the level of reliability of T-cell subsets in aging specimens |
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| المؤلفون: | N. Soucy, T. Ding, Janet K.A. Nicholson, Andrew D. Badley, N. C.Hawley Foss, Michèle Bergeron, Francis Mandy, S. Phaneuf |
| المصدر: | Cytometry. 50:53-61 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Time Factors, Light, CD8 Antigens, Lymphocyte, T cell, Biophysics, Gating, Biology, Immunophenotyping, Specimen Handling, Pathology and Forensic Medicine, Flow cytometry, Endocrinology, T-Lymphocyte Subsets, HIV Seronegativity, HIV Seropositivity, medicine, Enumeration, Humans, Scattering, Radiation, Lymphocytes, Protocol (science), Acquired Immunodeficiency Syndrome, medicine.diagnostic_test, Reproducibility of Results, Cell Biology, Hematology, Flow Cytometry, medicine.anatomical_structure, CD4 Antigens, Immunology, Leukocyte Common Antigens, Cytometry |
| الوصف: | BACKGROUND In the past decade, human immunodeficiency virus (HIV) lymphocyte immunophenotyping has evolved significantly. New fluorochromes, new multicolor reagents, enhanced instruments, and the capacity to provide absolute cell counts using the single-platform technique have all contributed to the reliability of T-cell subset measurements. In this study, four gating protocols were evaluated to select the most robust method for T-cell subset enumeration. Methods: Peripheral blood specimens from 21 HIV+ and 20 HIV− individuals were monitored up to 96 h. Aliquots of specimens were stored at room temperature and analyzed at 6 (baseline), 48, 72, and 96 h. Aliquots were stained with CD45-fluorescein isothiocyanate (FITC)/CD3PC5/CD4RD1/CD8ECD. Data analysis was performed with all four gating protocols. Results: Only with fresh blood did all protocols provide similar results. From samples that were 48 h old, the choice of gating strategy had a dramatic impact on immunophenotyping results. The largest deviations from baseline values occurred at 96 h and gating protocols that included dual light scatter gates provided the greatest shift of T-cell subset values over time. The gating protocols that were based exclusively on cell lineage-specific gates gave the most robust T-cell values up to 96 h. Conclusion: By selecting the appropriate gating protocol, the temporal integrity of specimens can be extended up to 4 days. Cytometry (Clin. Cytometry) 50:53–61, 2002. © 2002 Wiley-Liss, Inc. |
| تدمد: | 1097-0320 0196-4763 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0562f61b55bace7bd4d90db4fff340e2 https://doi.org/10.1002/cyto.10092 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....0562f61b55bace7bd4d90db4fff340e2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970320 01964763 |
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