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Probe-Free Identification of RNA Virus Variants with Point Mutations by Surface-Enhanced Raman Spectroscopy

التفاصيل البيبلوغرافية
العنوان: Probe-Free Identification of RNA Virus Variants with Point Mutations by Surface-Enhanced Raman Spectroscopy
المؤلفون: Su-jin Hong, Eungyeong Park, Yoon-ha Jang, Ji-yeon Shim, Yeonju Park, Sila Jin, Shuang Guo, Yeon-ju Kim, Min-jeong Son, Lei Chen, Kwang-il Lim, Young Mee Jung
المصدر: Analytical Chemistry. 94:17422-17430
بيانات النشر: American Chemical Society (ACS), 2022.
سنة النشر: 2022
مصطلحات موضوعية: Analytical Chemistry
الوصف: As observed in the COVID-19 pandemic, RNA viruses continue to rapidly evolve through mutations. In the absence of effective therapeutics, early detection of new severely pathogenic viruses and quarantine of infected people are critical for reducing the spread of the viral infections. However, conventional detection methods require a substantial amount of time to develop probes specific to new viruses, thereby impeding immediate response to the emergence of viral pathogens. In this study, we identified multiple types of viruses by obtaining the spectral fingerprint of their surface proteins with probe-free surface-enhanced Raman scattering (SERS). In addition, the SERS-based method can remarkably distinguish influenza virus variants with several surface protein point mutations from their parental strain. Principal component analysis (PCA) of the SERS spectra systematically captured the key Raman bands to distinguish the variants. Our results show that the combination of SERS and PCA can be a promising tool for rapid detection of newly emerging mutant viruses without a virus-specific probe.
تدمد: 1520-6882
0003-2700
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05a356f22d5fd7f41740a8494d573fce
https://doi.org/10.1021/acs.analchem.2c02912
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....05a356f22d5fd7f41740a8494d573fce
قاعدة البيانات: OpenAIRE
الوصف
تدمد:15206882
00032700