Prognosis of glioblastoma with faint MGMT methylation-specific PCR product
| العنوان: | Prognosis of glioblastoma with faint MGMT methylation-specific PCR product |
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| المؤلفون: | Yu-Shu Yen, Wan-You Guo, Donald Ming-Tak Ho, Yi-Chun Chang-Chien, Shih-Chieh Lin, Ming-Hsiung Chen, Chih-Yi Hsu, Hsiang-Ling Ho, Sanford Ping-Chuan Hsu |
| المصدر: | Journal of Neuro-Oncology. 122:179-188 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, Cancer Research, Pathology, medicine.medical_specialty, Adolescent, Concordance, Biology, Polymerase Chain Reaction, complex mixtures, law.invention, Immunoenzyme Techniques, Young Adult, law, parasitic diseases, medicine, Humans, Progression-free survival, Child, DNA Modification Methylases, neoplasms, Survival rate, Polymerase chain reaction, Aged, Neoplasm Staging, Aged, 80 and over, Brain Neoplasms, Tumor Suppressor Proteins, Methylation, DNA Methylation, Middle Aged, Prognosis, Molecular biology, Survival Rate, DNA Repair Enzymes, Neurology, Oncology, Case-Control Studies, Child, Preschool, DNA methylation, Pyrosequencing, Immunohistochemistry, Female, Neurology (clinical), Glioblastoma, Follow-Up Studies |
| الوصف: | Methylation-specific polymerase chain reaction (MSP) for the promoter methylation status of O(6)-methylguanine-DNA-methyltranferase (MGMT) gene theoretically provides a positive or negative result. However, the faint MSP product is difficult to interpret. The aim of this study was to evaluate the significance of faint MSP product in glioblastoma (GBM). Critical concentrations of methylated control DNA, i.e., 100, 1, 0.5 and 0 % were run parallel with 116 newly diagnosed GBMs in order to standardize the interpretation and to distinguish positive (+), equivocal (±), and negative (-; unmethylated) results. Cases with the faint MSP product and its intensity between those of 1 and 0.5 % DNA controls were considered equivocal (±). MGMT methylation quantifications were also determined by quantitative real-time MSP (qMSP) and pyrosequencing (PSQ), and protein expression was detected by immunohistochemistry. There were significant correlations between MSP and all the aforementioned studies. The concordance rates between the MSP+ and qMSP+ cases, as well as the MSP- and qMSP- cases were 100 %, and the MSP± cases comprised 76.5 % of qMSP+ cases and 23.5 % of qMSP- cases. PSQ study showed that heterogeneous methylation was more frequently encountered in the MSP± cases. Multivariate analyses disclosed that although the overall survival of the MSP± cases was indistinct from that of the MSP+ cases, its progression free survival was significantly worse and was indistinct from that of the MSP- cases. In conclusion, GBMs with faint MGMT MSP products should be distinguished from MSP+ cases as their behaviors were different. |
| تدمد: | 1573-7373 0167-594X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::064418e9345eacee63245179ce1fe5a1 https://doi.org/10.1007/s11060-014-1701-1 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....064418e9345eacee63245179ce1fe5a1 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 15737373 0167594X |
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