Evolution from adherent to suspension – systems biology of HEK293 cell line development
| العنوان: | Evolution from adherent to suspension – systems biology of HEK293 cell line development |
|---|---|
| المؤلفون: | Veronique Chotteau, Paul Varley, Ray Field, Jens Nielsen, Luigi Grassi, Diane Hatton, Thomas Svensson, Rasool Saghaleyni, Johan Rockberg, Magnus Lundqvist, Marco Giudici, Magdalena Malm |
| المصدر: | Scientific Reports Malm, M, Saghaleyni, R, Lundqvist, M, Giudici, M, Chotteau, V, Field, R, Varley, P G, Hatton, D, Grassi, L, Svensson, T, Nielsen, J & Rockberg, J 2020, ' Evolution from adherent to suspension: systems biology of HEK293 cell line development ', Scientific Reports, vol. 10, 18996 . https://doi.org/10.1038/s41598-020-76137-8 Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) |
| بيانات النشر: | Cold Spring Harbor Laboratory, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Molecular biology, Cell Culture Techniques, Gene Dosage, lcsh:Medicine, Biologics, Biology, Protein Engineering, Gene dosage, Article, Viral vector, Transcriptome, Cell Movement, Gene expression, Cell Adhesion, Humans, Metabolomics, Sequencing, Gene Regulatory Networks, lcsh:Science, Cell adhesion, Gene, Regulation of gene expression, Multidisciplinary, Gene Expression Profiling, lcsh:R, HEK 293 cells, Genomics, Computational biology and bioinformatics, Cell biology, Cellular component organization, Cholesterol, HEK293 Cells, Gene Expression Regulation, Cell culture, lcsh:Q |
| الوصف: | The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors. Despite the increased popularity of this strain we still have limited knowledge on the genetic composition of its derivatives. Here we present a genomic, transcriptomic and metabolic gene analysis of six of the most widely used HEK293 cell lines. Changes in gene copy and expression between industrial progeny cell lines and the original HEK293 were associated with cellular component organization, cell motility and cell adhesion. Changes in gene expression between adherent and suspension derivatives highlighted switching in cholesterol biosynthesis and expression of five key genes (RARG, ID1, ZIC1, LOX and DHRS3), a pattern validated in 63 human adherent or suspension cell lines of other origin. |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::065ccb2419a777e8196c0b29f5c6f92c https://doi.org/10.1101/2020.01.29.924894 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....065ccb2419a777e8196c0b29f5c6f92c |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |