Resonance Raman studies of iron-only hydrogenases
| العنوان: | Resonance Raman studies of iron-only hydrogenases |
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| المؤلفون: | Drozdzewski Pm, He Sh, L. E. Mortenson, Harry D. Peck, T.V. Morgan, Juszczak A, Jean LeGall, Weiguang Fu, DerVartanian Dv, Michael W. W. Adams |
| المصدر: | Biochemistry. 32:4813-4819 |
| بيانات النشر: | American Chemical Society (ACS), 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Iron-Sulfur Proteins, Hydrogenase, Hydrogen, Iron, Resonance Raman spectroscopy, Inorganic chemistry, chemistry.chemical_element, Iron–sulfur cluster, Spectrum Analysis, Raman, Biochemistry, symbols.namesake, chemistry.chemical_compound, Desulfovibrio vulgaris, Clostridium, Gram-Negative Anaerobic Bacteria, biology, Chemistry, Resonance, biology.organism_classification, Crystallography, Thermotoga maritima, symbols, Raman spectroscopy, Sulfur |
| الوصف: | The nature of the iron-sulfur clusters in oxidized and reduced forms of Fe-only hydrogenases from Desulfovibrio vulgaris, Thermotoga maritima, and Clostridium pasteurianum has been investigated by resonance Raman spectroscopy. The results indicate the presence of ferredoxin-like [4Fe-4S]2+,+ and [2Fe-2S]2+,+ clusters in both T. maritima hydrogenase and C. pasteurianum hydrogenase I, but only [4Fe-4S]2+,+ clusters in D. vulgaris hydrogenase. This necessitates a reevaluation of the iron-sulfur cluster composition of C. pasteurianum hydrogenase I and indicates that the resonance Raman bands in the oxidized hydrogenase that were previously attributed to the hydrogen activating center [Macor, K. A., Czernuszewicz, R. S., Adams, M. W. W., & Spiro, T. G. (1987) J. Biol. Chem. 262, 9945-9947] arise from an indigenous [2Fe-2S]2+ cluster. No resonance Raman bands that could be uniquely attributed to the oxidized or reduced hydrogen activating center were observed. This suggests that the hydrogen activating center is a novel Fe center that is unrelated to any known type of Fe-S cluster. |
| تدمد: | 1520-4995 0006-2960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06e06dffa3e51c3454d926f5ed1ec33d https://doi.org/10.1021/bi00069a016 |
| رقم الأكسشن: | edsair.doi.dedup.....06e06dffa3e51c3454d926f5ed1ec33d |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15204995 00062960 |
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