Mannitol Does Not Enhance Tobramycin Killing of Pseudomonas aeruginosa in a Cystic Fibrosis Model System of Biofilm Formation
العنوان: | Mannitol Does Not Enhance Tobramycin Killing of Pseudomonas aeruginosa in a Cystic Fibrosis Model System of Biofilm Formation |
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المؤلفون: | Paul Mastoridis, Katherine E. Price, Wesley Hebert, George A. O'Toole, Giulia Orazi, Kathryn L. Ruoff |
المصدر: | PLoS ONE PLoS ONE, Vol 10, Iss 10, p e0141192 (2015) |
بيانات النشر: | Public Library of Science, 2015. |
سنة النشر: | 2015 |
مصطلحات موضوعية: | Multidrug tolerance, Cystic Fibrosis, medicine.drug_class, Antibiotics, lcsh:Medicine, medicine.disease_cause, Cystic fibrosis, Microbiology, medicine, Tobramycin, Humans, Mannitol, Pseudomonas Infections, lcsh:Science, Lung, Multidisciplinary, Chemistry, Pseudomonas aeruginosa, Aminoglycoside, lcsh:R, Biofilm, medicine.disease, Mucus, Biofilms, lcsh:Q, medicine.drug, Research Article |
الوصف: | Cystic Fibrosis (CF) is a human genetic disease that results in the accumulation of thick, sticky mucus in the airways, which results in chronic, life-long bacterial biofilm infections that are difficult to clear with antibiotics. Pseudomonas aeruginosa lung infection is correlated with worsening lung disease and P. aeruginosa transitions to an antibiotic tolerant state during chronic infections. Tobramycin is an aminoglycoside currently used to combat lung infections in individuals with CF. While tobramycin is effective at eradicating P. aeruginosa in the airways of young patients, it is unable to completely clear the chronic P. aeruginosa infections in older patients. A recent report showed that co-addition of tobramycin and mannitol enhanced killing of P. aeruginosa grown in vitro as a biofilm on an abiotic surface. Here we employed a model system of bacterial biofilms formed on the surface of CF-derived airway cells to determine if mannitol would enhance the antibacterial activity of tobramycin against P. aeruginosa grown on a more clinically relevant surface. Using this model system, which allows the growth of robust biofilms with high-level antibiotic tolerance analogous to in vivo biofilms, we were unable to find evidence for enhanced antibacterial activity of tobramycin with the addition of mannitol, supporting the observation that this type of co-treatment failed to reduce the P. aeruginosa bacterial load in a clinical setting. |
اللغة: | English |
تدمد: | 1932-6203 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::073c0ee11fc528b020db2e085641a952 http://europepmc.org/articles/PMC4624634 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....073c0ee11fc528b020db2e085641a952 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19326203 |
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