Scaffold-mediated gating of Cdc42 signalling flux
| العنوان: | Scaffold-mediated gating of Cdc42 signalling flux |
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| المؤلفون: | Craig R. Braun, Derek McCusker, Aurelie Massoni-Laporte, Laure Bataille, Steven P. Gygi, Caner Ünlü, Floriane Saint Arramon, Péter Rapali, Romain Mitteau |
| المساهمون: | Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Chemistry Department, Istanbul Technical University, Istanbul 34469, Turkey, Laboratoire de Chimie des Polymères Organiques (LCPO), Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Ecole Nationale Supérieure de Chimie, de Biologie et de Physique (ENSCBP)-Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC), Department of cell biology, Harvard Medical School [Boston] (HMS), University of California [Santa Cruz] (UCSC), University of California |
| المصدر: | eLife eLife, eLife Sciences Publication, 2017, 6, ⟨10.7554/eLife.25257⟩ eLife, Vol 6 (2017) |
| بيانات النشر: | eLife Sciences Publications, Ltd, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Scaffold protein, Saccharomyces cerevisiae Proteins, Intravital Microscopy, QH301-705.5, Science, Short Report, S. cerevisiae, CDC42, GTPase, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Saccharomyces cerevisiae, Biology, environment and public health, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Chloride Channels, Cell polarity, Biology (General), ComputingMilieux_MISCELLANEOUS, Adaptor Proteins, Signal Transducing, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae, Microscopy, General Immunology and Microbiology, Kinase, General Neuroscience, Rho GTPase, General Medicine, Cell Biology, 3. Good health, Cell biology, enzymes and coenzymes (carbohydrates), cell polarity, 030104 developmental biology, Medicine, Phosphorylation, Guanine nucleotide exchange factor, Guanosine Triphosphate, biological phenomena, cell phenomena, and immunity, signaling, Flux (metabolism), Signal Transduction |
| الوصف: | Scaffold proteins modulate signalling pathway activity spatially and temporally. In budding yeast, the scaffold Bem1 contributes to polarity axis establishment by regulating the GTPase Cdc42. Although different models have been proposed for Bem1 function, there is little direct evidence for an underlying mechanism. Here, we find that Bem1 directly augments the guanine exchange factor (GEF) activity of Cdc24. Bem1 also increases GEF phosphorylation by the p21-activated kinase (PAK), Cla4. Phosphorylation abrogates the scaffold-dependent stimulation of GEF activity, rendering Cdc24 insensitive to additional Bem1. Thus, Bem1 stimulates GEF activity in a reversible fashion, contributing to signalling flux through Cdc42. The contribution of Bem1 to GTPase dynamics was borne-out by in vivo imaging: active Cdc42 was enriched at the cell pole in hypophosphorylated cdc24 mutants, while hyperphosphorylated cdc24 mutants that were resistant to scaffold stimulation displayed a deficit in active Cdc42 at the pole. These findings illustrate the self-regulatory properties that scaffold proteins confer on signalling pathways. DOI: http://dx.doi.org/10.7554/eLife.25257.001 |
| اللغة: | English |
| تدمد: | 2050-084X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::076584e132b85f68501e7bafe3b6a15a http://europepmc.org/articles/PMC5386590 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....076584e132b85f68501e7bafe3b6a15a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2050084X |
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