NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis
| العنوان: | NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis |
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| المؤلفون: | Vanathy Easwaran, Masanori Okamoto, Ryoichi Yano, Anne Krapp, Yunchen Gong, Steven J. Rothstein, Dawei Yan, Akira Endo, Yong-Mei Bi, David S. Guttman, Vivian Chau, Matthew Ierullo, Nicolas Provart, Eiji Nambara, Mitsuhiro Kimura, Asher Pasha |
| المساهمون: | Department of Cell & Systems Biology, University of Toronto, JST, PRESTO, Tottori University, Shimane University, Université de Tsukuba = University of Tsukuba, Centre for the Analysis of Genome Evolution and Function, Department of Molecular and Cellular Biology, University of Guelph, Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, NSERC [RGPIN 355784, RGPIN-2014-03621], JST PRESTO [11105], Labex Saclay Plant Sciences [ANR-10-LABX-0040-SPS], Nambara, Eiji |
| المصدر: | Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016) Nature Communications Nature Communications, Nature Publishing Group, 2016, 7, pp.1-11. ⟨10.1038/ncomms13179⟩ Nature Communications (7), 1-11. (2016) |
| بيانات النشر: | NATURE PUBLISHING GROUP, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, [SDV]Life Sciences [q-bio], Science, Mutant, Arabidopsis, General Physics and Astronomy, Germination, Biology, 01 natural sciences, Genetic analysis, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, Cytochrome P-450 Enzyme System, Plant Growth Regulators, Nitrate, Gene Expression Regulation, Plant, Botany, [SDV.IDA]Life Sciences [q-bio]/Food engineering, Protein Isoforms, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Promoter Regions, Genetic, Abscisic acid, Conserved Sequence, Plant Proteins, chemistry.chemical_classification, Nitrates, Multidisciplinary, Arabidopsis Proteins, Catabolism, fungi, Gene Expression Regulation, Developmental, food and beverages, General Chemistry, biology.organism_classification, Cell biology, 030104 developmental biology, Enzyme, chemistry, Seeds, Abscisic Acid, Transcription Factors, 010606 plant biology & botany |
| الوصف: | Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling. Nitrate stimulates seed germination in many plant species. Here, Yan et al. show that the Arabidopsis transcription factor NIN-like protein 8 is required to stimulate germination in response to nitrate and induces expression of an enzyme involved in ABA catabolism. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2041-1723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::076adcbadcfd00d1f44f66fa74255c7c http://ir.lib.shimane-u.ac.jp/53469 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....076adcbadcfd00d1f44f66fa74255c7c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
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