Characterizing the radioresponse of pluripotent and multipotent human stem cells
| العنوان: | Characterizing the radioresponse of pluripotent and multipotent human stem cells |
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| المؤلفون: | Jessica Bahari-Kashani, Katherine K. Tran, Jan Strnadel, Mary L. Lan, Munjal M. Acharya, Neal H. Patel, Erich Giedzinski, Charles L. Limoli |
| المصدر: | PLoS ONE, Vol 7, Iss 12, p e50048 (2012) PLoS ONE |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Time Factors, DNA Repair, medicine.medical_treatment, Cellular differentiation, lcsh:Medicine, Apoptosis, Molecular cell biology, 0302 clinical medicine, Neural Stem Cells, Induced pluripotent stem cell, lcsh:Science, Cellular Stress Responses, 0303 health sciences, Multidisciplinary, Cell Death, Stem Cells, Statistics, Cell Cycle, Cell Differentiation, Stem-cell therapy, Immunohistochemistry, Cell biology, Nucleic acids, Endothelial stem cell, 030220 oncology & carcinogenesis, Cellular Types, Stem cell, Oxidation-Reduction, Research Article, Adult stem cell, Cell Survival, Induced Pluripotent Stem Cells, Biostatistics, Biology, Cell Line, 03 medical and health sciences, medicine, Humans, Embryonic Stem Cells, 030304 developmental biology, lcsh:R, Radiobiology, DNA, Embryonic stem cell, Kinetics, Oxidative Stress, Multipotent Stem Cell, Immunology, lcsh:Q, Mathematics, Developmental Biology, DNA Damage |
| الوصف: | The potential capability of stem cells to restore functionality to diseased or aged tissues has prompted a surge of research, but much work remains to elucidate the response of these cells to genotoxic agents. To more fully understand the impact of irradiation on different stem cell types, the present study has analyzed the radioresponse of human pluripotent and multipotent stem cells. Human embryonic stem (ES) cells, human induced pluripotent (iPS) cells, and iPS-derived human neural stem cells (iPS-hNSCs) cells were irradiated and analyzed for cell survival parameters, differentiation, DNA damage and repair and oxidative stress at various times after exposure. While irradiation led to dose-dependent reductions in survival, the fraction of surviving cells exhibited dose-dependent increases in metabolic activity. Irradiation did not preclude germ layer commitment of ES cells, but did promote neuronal differentiation. ES cells subjected to irradiation exhibited early apoptosis and inhibition of cell cycle progression, but otherwise showed normal repair of DNA double-strand breaks. Cells surviving irradiation also showed acute and persistent increases in reactive oxygen and nitrogen species that were significant at nearly all post-irradiation times analyzed. We suggest that stem cells alter their redox homeostasis to adapt to adverse conditions and that radiation-induced oxidative stress plays a role in regulating the function and fate of stem cells within tissues compromised by radiation injury. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0771cab3e9c5acae7544443c1183ef05 http://europepmc.org/articles/PMC3522689?pdf=render |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0771cab3e9c5acae7544443c1183ef05 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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