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3D Printing-Enabled DNA Extraction for Long-Read Genomics

التفاصيل البيبلوغرافية
العنوان: 3D Printing-Enabled DNA Extraction for Long-Read Genomics
المؤلفون: Kevin D. Dorfman, Paridhi Agrawal, Jeffrey G. Reifenberger
المصدر: ACS Omega
ACS Omega, Vol 5, Iss 33, Pp 20817-20824 (2020)
سنة النشر: 2020
مصطلحات موضوعية: General Chemical Engineering, Genomics, General Chemistry, Computational biology, Biology, DNA extraction, Article, Chemistry, chemistry.chemical_compound, Gene mapping, chemistry, Genomic information, Nanopore sequencing, QD1-999, DNA
الوصف: Long-read genomics technologies such as nanopore sequencing and genome mapping in nanochannels extract genomic information in the kilobase to megabase pair range from single DNA molecules, thereby overcoming read-length limitations in next-generation DNA sequencing. Long-read technologies start with long DNA molecules as the input and thus benefit from universal sample preparation methods that are fast and shear-free and present a scope of automation and direct upstream integration. We describe a 3D printing-assisted poly(dimethylysiloxane)-based DNA sample preparation device, where diffusive chemical lysis followed by electrophoresis produces circa 100 ng of long DNA directly from cells with less than 5 min of labor. Assessment of the product DNA by confinement in nanochannels reveals that the DNA sizes are commensurate with the requirements for long-read single-molecule technologies. Microfluidics not only expedites sample preparation, but also offers the opportunity for integration with genomics technologies to eliminate DNA fragmentation and loss during transfer to the genomic device.
تدمد: 2470-1343
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07f3d22c5b899324be2f6a7e5c524d0e
https://pubmed.ncbi.nlm.nih.gov/32875216
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....07f3d22c5b899324be2f6a7e5c524d0e
قاعدة البيانات: OpenAIRE
الوصف
تدمد:24701343