The Src-like adaptor protein regulates PDGF-induced actin dorsal ruffles in a c-Cbl-dependent manner
| العنوان: | The Src-like adaptor protein regulates PDGF-induced actin dorsal ruffles in a c-Cbl-dependent manner |
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| المؤلفون: | Anthony Boureux, Valérie Simon, Serge Roche, Audrey Sirvent, Cédric Leroy |
| المساهمون: | Centre de recherche en Biologie Cellulaire (CRBM), Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1) |
| المصدر: | Oncogene Oncogene, Nature Publishing Group, 2008, 27 (24), pp.3494-500. ⟨10.1038/sj.onc.1211011⟩ |
| بيانات النشر: | HAL CCSD, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | MESH: Signal Transduction, Cancer Research, MESH: rac GTP-Binding Proteins, SRC Family Tyrosine Kinase, SH2 domain, environment and public health, Mice, Receptors, Platelet-Derived Growth Factor, MESH: Animals, MESH: Proto-Oncogene Proteins c-cbl, MESH: Receptors, Platelet-Derived Growth Factor, Proto-Oncogene Proteins c-cbl, Phosphorylation, STAT3, Lung, Platelet-Derived Growth Factor, 0303 health sciences, biology, 030302 biochemistry & molecular biology, MESH: Platelet-Derived Growth Factor, Signal transducing adaptor protein, Cell biology, rac GTP-Binding Proteins, src-Family Kinases, Tyrosine kinase, Platelet-derived growth factor receptor, Proto-oncogene tyrosine-protein kinase Src, Signal Transduction, Proto-Oncogene Proteins pp60(c-src), Mitosis, MESH: Binding, Competitive, macromolecular substances, MESH: Actins, Binding, Competitive, 03 medical and health sciences, Genetics, Animals, Point Mutation, MESH: Lung, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, MESH: Mice, 030304 developmental biology, MESH: Point Mutation, MESH: Phosphorylation, MESH: Proto-Oncogene Proteins pp60(c-src), Fibroblasts, MESH: Mitosis, Actins, enzymes and coenzymes (carbohydrates), MESH: src-Family Kinases, MESH: Fibroblasts, Cancer research, biology.protein, NIH 3T3 Cells, MESH: NIH 3T3 Cells |
| الوصف: | The Src-like adaptor protein (SLAP) belongs to the subfamily of adapter proteins that negatively regulate cellular signalling initiated by tyrosine kinases. SLAP has a unique, myristylated N-terminus, followed by SH3 and SH2 domains with high homology to Src family tyrosine kinases (SFK) and a unique C-terminal tail, which is important for c-Cbl binding. We have previously shown that SLAP negatively regulates platelet-derived growth factor (PDGF)-induced mitogenesis in fibroblasts and we now report that it regulates F-actin assembly for dorsal ruffles formation. c-Cbl mediated SLAP inhibition towards actin remodelling. Moreover, SLAP enhanced PDGF-induced c-Cbl phosphorylation by SFK. In contrast, SLAP mitogenic inhibition was not mediated by c-Cbl, but it rather involved a competitive mechanism with SFK for PDGF-receptor (PDGFR) association and mitogenic signalling. Accordingly, phosphorylation of the Src mitogenic substrates Stat3 and Shc were reduced by SLAP. Thus, we concluded that SLAP regulates PDGFR signalling by two independent mechanisms: a competitive mechanism for PDGF-induced Src mitogenic signalling and a non-competitive mechanism for dorsal ruffles formation mediated by c-Cbl. |
| اللغة: | English |
| تدمد: | 0950-9232 1476-5594 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0817ab5a7a7aa63c85e4de734e5e527b https://hal.archives-ouvertes.fr/hal-00211547/file/Sirvent-SLAP-Oncogene.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0817ab5a7a7aa63c85e4de734e5e527b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09509232 14765594 |
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