Molecular identification of burn spot disease syndrome agent Fusarium avenaceum in Turkish crayfish Pontastacus leptodactylus populations
| العنوان: | Molecular identification of burn spot disease syndrome agent Fusarium avenaceum in Turkish crayfish Pontastacus leptodactylus populations |
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| المؤلفون: | Süleyman Akhan, Büşra Taştan |
| المصدر: | Diseases of Aquatic Organisms. 144:33-40 |
| بيانات النشر: | Inter-Research Science Center, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, Veterinary medicine, Turkey, biology, Astacoidea, Aquatic Science, biology.organism_classification, Crayfish, 010603 evolutionary biology, 01 natural sciences, Leptodactylus, Fusarium avenaceum, 03 medical and health sciences, 030104 developmental biology, Fusarium, GenBank, Pontastacus leptodactylus, Animals, Potato dextrose agar, Internal transcribed spacer, DNA, Fungal, Pathogen, Ecology, Evolution, Behavior and Systematics |
| الوصف: | In this study, we aimed to isolate and identify the causative agent of burn spot disease syndrome from crayfish populations within Turkish water bodies. Crayfish Pontastacus leptodactylus samples were captured from Bayramşah Reservoir, Karaidemir Reservoir and Keban Reservoir (Turkey). Fungi samples were taken from symptomatic crayfish and cultured in potato dextrose agar, and DNAs were extracted. Species-specific PCR primers (FAF1 and FAR) were used in order to amplify the internal transcribed spacer (ITS) region of fungal DNA. Sequences obtained from the amplified regions were analyzed using GenBank and identified. As a result, a molecular diagnosis of the fungi samples was made, and the pathogen was identified as Fusarium avenaceum. In addition, a challenge test with healthy crayfish was conducted to evaluate the pathogenicity of the fungi. F. avenaceum was reisolated from experimentally infested individuals, and thusly we confirmed the disease. It was concluded that F. avenaceum causes burn spot disease syndrome in P. leptodactylus. |
| تدمد: | 1616-1580 0177-5103 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0852dbb91c0510415709f4a067232b94 https://doi.org/10.3354/dao03570 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....0852dbb91c0510415709f4a067232b94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16161580 01775103 |
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