Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer
| العنوان: | Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer |
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| المؤلفون: | Hui Zhang, Dechun Li, Yue-yu Chen, Jinlong Yan, Pei-dong Shi, Songbing He, Kaiping Zhou, Boshun Wan, Wei-qiang Yang |
| المصدر: | International Journal of Oncology |
| بيانات النشر: | D.A. Spandidos, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Cancer Research, Epithelial-Mesenchymal Transition, Myosin Light Chains, Cell, Mice, Nude, colorectal cancer, Biology, migration, Metastasis, myosin light chain, 03 medical and health sciences, Mice, Profilins, 0302 clinical medicine, Cell Movement, Cell Line, Tumor, medicine, Animals, Humans, Epithelial–mesenchymal transition, Phosphorylation, Cytoskeleton, Aged, Cell Proliferation, Mice, Inbred BALB C, Oncogene, Cancer, Articles, Cell cycle, Middle Aged, Actin cytoskeleton, medicine.disease, Molecular medicine, Xenograft Model Antitumor Assays, Up-Regulation, Gene Expression Regulation, Neoplastic, 030104 developmental biology, medicine.anatomical_structure, Oncology, 030220 oncology & carcinogenesis, Cancer research, profilin 2, Female, Colorectal Neoplasms, Signal Transduction |
| الوصف: | Profilin 2 (PFN2) functions as an actin cytoskeleton regulator and serves an important role in cell motility. However, a role for PFN2 in the progression of colorectal cancer (CRC), particularly in metastasis, has yet to be clarified. The aim of the present study was to investigate whether PFN2 served specific roles in the progression of human CRC. The results demonstrated that PFN2 was differentially expressed in CRC tissues and cell lines by reverse transcription-quantitative polymerase chain reaction and western blotting. PFN2 expression was also negatively associated with the degree of tumor metastasis. Low PFN2 expression in CRC cells was related with enhanced epithelial-mesenchymal transition (EMT) and, in turn, may increase migratory capabilities. Overexpression of PFN2 in CRC cell lines with a low level of endogenous PFN2 inhibited the EMT process, as well as the associated migration; in addition, myosin light chain (MLC) phosphorylation was upregulated. Inhibition of MLC phosphorylation attenuated the inhibition of EMT and cell migratory abilities induced by PFN2 overexpression in CRC cell lines, the results suggested that PFN2 may suppress cancer EMT and the subsequent metastasis by regulating cytoskeletal reorganization. These results demonstrated that PFN2 may serve a suppressive role in the metastasis of CRC and therefore may provide a new potential target for cancer therapeutics. |
| اللغة: | English |
| تدمد: | 1791-2423 1019-6439 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08b125d485631e16051948a485d1b867 http://europepmc.org/articles/PMC6065425 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....08b125d485631e16051948a485d1b867 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912423 10196439 |
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