Tenascin C regulates cancer cell glycolysis and tumor progression in prostate cancer

التفاصيل البيبلوغرافية
العنوان: Tenascin C regulates cancer cell glycolysis and tumor progression in prostate cancer
المؤلفون: Yongri Qian, Xingzhe Liu, Ying Feng, Xiaogang Li, Yanhua Xuan
المصدر: International journal of urology : official journal of the Japanese Urological AssociationReferences. 29(6)
سنة النشر: 2022
مصطلحات موضوعية: Male, Phosphatidylinositol 3-Kinases, Glucose, Receptors, Androgen, Urology, Cell Line, Tumor, Lactates, NF-kappa B, Humans, Prostatic Neoplasms, Tenascin, Glycolysis
الوصف: Tenascin C is a potential biomarker of cancer-associated fibroblasts and has been significantly associated with poor prognosis in patients with prostate cancer. However, the effects of Tenascin C in prostate cancer cell glycolysis largely remain unclear. Thus, this study aimed to investigate the Tenascin C expression in prostate cancer and its correlation to glycolysis-related protein and gene expression, clinicopathological parameters, and survival of patients.We performed immunohistochemical staining for Tenascin C in 141 cases of primary prostate cancer. Based on public data sets, we explored the association of Tenascin C with angiogenesis-related genes, M2 macrophage-related gene, androgen receptor levels, PI3K/AKT/NF-κB pathway genes, and glycolytic enzyme expression. The glucose uptake, lactate production, and glycolytic enzyme levels were detected by glycolysis assay and western blotting.Our results showed that Tenascin C expression is upregulated in prostate cancer tissues compared with benign prostatic hyperplasia tissues. High Tenascin C expression in prostate cancer cells was positively associated with lymph node metastasis, advanced clinical stage, the expression of CD105, CD206, and androgen receptor levels. The Kaplan-Meier curves showed a significant association of Tenascin C expression with the patient's overall survival. Tenascin C expression was positively associated with PI3K p85, pAKT-ser308, and NF-κB p65 protein expression in prostate cancer samples. Moreover, siRNA-mediated knockdown of Tenascin C expression inhibited cell glucose uptake, lactate production, and glycolytic-enzyme expression in prostate cancer cells in vitro.Together, our findings suggest that Tenascin C is a prognostic marker for patients with prostate cancer and that its effects might be mediated via regulation of the glycolysis process of prostate cancer cells.
تدمد: 1442-2042
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0912731b25f454f5b278e528b4108261
https://pubmed.ncbi.nlm.nih.gov/35368121
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....0912731b25f454f5b278e528b4108261
قاعدة البيانات: OpenAIRE