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Identification of a Novel Fusion Gene, FAM174A-WWC1, in Early-Onset Colorectal Cancer: Establishment and Characterization of Four Human Cancer Cell Lines from Early-Onset Colorectal Cancers

التفاصيل البيبلوغرافية
العنوان: Identification of a Novel Fusion Gene, FAM174A-WWC1, in Early-Onset Colorectal Cancer: Establishment and Characterization of Four Human Cancer Cell Lines from Early-Onset Colorectal Cancers
المؤلفون: Min Jung Kim, Ji Won Park, Seung-Yong Jeong, Soon Chan Kim, Rumi Shin, Ha Young Seo, Ja-Lok Ku
المصدر: Translational Oncology, Vol 12, Iss 9, Pp 1185-1195 (2019)
Translational Oncology
بيانات النشر: Elsevier, 2019.
سنة النشر: 2019
مصطلحات موضوعية: 0301 basic medicine, YAP1, Original article, Cancer Research, Mutation, Colorectal cancer, Incidence (epidemiology), Cancer, Biology, medicine.disease, medicine.disease_cause, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282, Fusion gene, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Oncology, Cell culture, 030220 oncology & carcinogenesis, medicine, Cancer research, Fibroblast
الوصف: Colorectal cancer (CRC) is the third most common cancer diagnosed worldwide and represents the second most common cause of all cancer-related deaths in Korea. Although epidemiological data indicate a sharp increase in the incidence of CRC among individuals older than 50 years, more than 10% of CRCs occur before reaching 50. These are known as early-onset CRCs (EOCRCs) and are likely to be suggestive of hereditary predisposition. However, known familial CRC syndromes account for only 20% of genetic aberrations of EOCRC, and the remaining 80% are still in question. Therefore, we aimed to establish reproducible biological resources and contribute to expand the mutation database of EOCRC. Four cell lines derived from the original tumor mass of CRC patients diagnosed under age 30 years were established, and next-generation sequencing technique was used to identify the genetic features of EOCRC. We have identified one novel fusion gene, FAM174A-WWC1, and analyzed its functional role. The induction of FAM174A-WWC1 to normal fibroblast caused alternations in cellular morphology as well as intercellular expression of E-cadherin and N-cadherin. Moreover, WWC1 carrying the fused FAM174A domain not only abrogated the membrane expression of YAP1 but also significantly increased the levels of nucleic YAP1. As a result, the FAM174A-WWC1 expression increased the oncogenic capacity and invasiveness of normal fibroblasts, which suggests its role as a potential driver mutation of EOCRC.
اللغة: English
تدمد: 1936-5233
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09132fc86753dfecbe616f2c967bd888
http://www.sciencedirect.com/science/article/pii/S1936523319300956
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....09132fc86753dfecbe616f2c967bd888
قاعدة البيانات: OpenAIRE
الوصف
تدمد:19365233