Anti-oxidants correct disturbance of redox enzymes in the hearts of rat fetuses with congenital diaphragmatic hernia
| العنوان: | Anti-oxidants correct disturbance of redox enzymes in the hearts of rat fetuses with congenital diaphragmatic hernia |
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| المؤلفون: | Vicente Andreu-Fernández, Rosa Aras-López, Leopoldo Martinez, Juan A. Tovar, L Almeida |
| المصدر: | PEDIATRIC SURGERY INTERNATIONAL r-FSJD: Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu Fundació Sant Joan de Déu r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu instname |
| بيانات النشر: | SPRINGER, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, GPX1, Anti-oxidants, Apocynin, Cardiac malformations, Congenital diaphragmatic hernia, Epigallocatechin-3-gallate, Nitrofen, Pulmonary hypertension, Redox metabolism, Antioxidants, Catechin, Rats, Sprague-Dawley, chemistry.chemical_compound, Glutathione Peroxidase GPX1, Pregnancy, heterocyclic compounds, biology, NOX4, food and beverages, General Medicine, Catalase, NADPH Oxidase 4, Anesthesia, NADPH Oxidase 2, cardiovascular system, NADPH Oxidase 1, Female, circulatory and respiratory physiology, inorganic chemicals, medicine.medical_specialty, SOD2, complex mixtures, 03 medical and health sciences, Internal medicine, medicine, Animals, RNA, Messenger, Fetus, Glutathione Peroxidase, business.industry, Superoxide Dismutase, Myocardium, Congenital diaphragmatic hernia, Acetophenones, Hydrogen Peroxide, Nitrofen, medicine.disease, Rats, Disease Models, Animal, 030104 developmental biology, Endocrinology, chemistry, Pediatrics, Perinatology and Child Health, Apocynin, biology.protein, Surgery, sense organs, business, Hernias, Diaphragmatic, Congenital |
| الوصف: | To evaluate if the redox system is unbalanced in the hearts of nitrofen-induced congenital diaphragmatic hernia (CDH) animals and to study the possible preventive effects of two anti-oxidant treatments, apocynin and epigallocatechin-3-gallate (EGCG). Adult rats were divided into four groups. Group 1: rats received only vehicle on day E9.5. Group 2: rats received 100 mg nitrofen on day E9.5. Group 3: 1 month before mating rats received apocynin 1.5 mM and, when pregnant, 100 mg nitrofen on day E9.5. Group 4: same than group 3 but with EGCG 30 mg/kg. All fetuses were recovered at term and the hearts were processed. Nox activity and mRNA levels of Nox1, Nox2, Nox4, SOD1, SOD2, SOD3, catalase, and GPX1 were analyzed. Nox, SOD, and Catalase activity and H2O2 production were also evaluated. Nox activity, H2O2 production and Nox1, Nox2, and Nox4 mRNA levels were increased in the hearts of fetuses with CDH. There were no changes in SOD1 levels, whereas those of SOD2, SOD3, catalase, and GPX1 mRNA were decreased. Apocynin and EGCG treatments attenuated the increment of Nox and SOD activities and H2O2 production was only decreased by apocynin. These findings suggest a possible preventive effect on the abnormal redox metabolism of anti-oxidant treatments in the hearts from rat fetuses with CDH. If the same occurs in humans, it could represent a potential tool in future prenatal treatment. |
| تدمد: | 0179-0358 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09415d59925fc3159c81c711b3670a18 http://fundanet.fsjd.org/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=13130 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....09415d59925fc3159c81c711b3670a18 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 01790358 |
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