A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice
| العنوان: | A DNA Vaccine Protects Human Immune Cells against Zika Virus Infection in Humanized Mice |
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| المؤلفون: | Xuequn Xu, Guohua Yi, Hua Guo, Sean Petersen, Premlata Shankar, Sojan Abraham, N. Manjunath, Nora Ortega |
| المصدر: | EBioMedicine EBioMedicine, Vol 25, Iss C, Pp 87-94 (2017) |
| بيانات النشر: | Elsevier, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, DNA vaccine, Cell type, Humanized DRAG mice, 030231 tropical medicine, lcsh:Medicine, Enzyme-Linked Immunosorbent Assay, Mice, Transgenic, General Biochemistry, Genetics and Molecular Biology, Neutralization, Virus, Zika virus, DNA vaccination, Zika virus (ZIKV), 03 medical and health sciences, Mice, 0302 clinical medicine, Immune system, Viral Envelope Proteins, Vaccines, DNA, Animals, Humans, Neutralizing antibody, lcsh:R5-920, biology, Zika Virus Infection, lcsh:R, Viral Vaccines, General Medicine, Zika Virus, biology.organism_classification, Virology, Antibodies, Neutralizing, 030104 developmental biology, Immunology, biology.protein, Antibody, lcsh:Medicine (General), B cell infection, Research Paper |
| الوصف: | A DNA vaccine encoding prM and E protein has been shown to induce protection against Zika virus (ZIKV) infection in mice and monkeys. However, its effectiveness in humans remains undefined. Moreover, identification of which immune cell types are specifically infected in humans is unclear. We show that human myeloid cells and B cells are primary targets of ZIKV in humanized mice. We also show that a DNA vaccine encoding full length prM and E protein protects humanized mice from ZIKV infection. Following administration of the DNA vaccine, humanized DRAG mice developed antibodies targeting ZIKV as measured by ELISA and neutralization assays. Moreover, following ZIKV challenge, vaccinated animals presented virtually no detectable virus in human cells and in serum, whereas unvaccinated animals displayed robust infection, as measured by qRT-PCR. Our results utilizing humanized mice show potential efficacy for a targeted DNA vaccine against ZIKV in humans. Highlights • Zika DNA vaccine elicits protective antibody response in humanized DRAG mice. • Human myeloid cells and B cells are targets of Zika virus. A Zika virus vaccine requires testing in human immune cells to determine its effectiveness. In this communication, we report that a DNA vaccine encoding Zika prM and E protein is able to elicit protective neutralizing antibodies against Zika virus in humanized DRAG mice, and that human myeloid cells and B cells are primary targets of Zika virus. |
| اللغة: | English |
| تدمد: | 2352-3964 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09b3a51f261000c6d25882ce2f1b482f http://europepmc.org/articles/PMC5704055 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....09b3a51f261000c6d25882ce2f1b482f |
| قاعدة البيانات: | OpenAIRE |
Full Text via ClinicalKey | تدمد: | 23523964 |
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