Human B cells express the orphan chemokine receptor CRAM-A/B in a maturation-stage-dependent and CCL5-modulated manner
| العنوان: | Human B cells express the orphan chemokine receptor CRAM-A/B in a maturation-stage-dependent and CCL5-modulated manner |
|---|---|
| المؤلفون: | Ingrid U. Schraufstatter, Tanja Nicole Hartmann, Andrea Diefenbacher, Marek Honczarenko, Susann Ewers, Meike Burger, Marion Leick |
| المصدر: | Immunology. 125:252-262 |
| بيانات النشر: | Wiley, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | CCR1, Chemokine receptor CCR5, Immunology, C-C chemokine receptor type 6, Receptors, CCR, Chemokine receptor, Stress Fibers, Humans, Protein Isoforms, Immunology and Allergy, Calcium Signaling, CXC chemokine receptors, Phosphorylation, Chemokine CCL5, Cells, Cultured, Mitogen-Activated Protein Kinase 1, B-Lymphocytes, Mitogen-Activated Protein Kinase 3, biology, Cell Differentiation, Original Articles, Cell biology, Chemotaxis, Leukocyte, biology.protein, XCL2, CC chemokine receptors, Signal Transduction, CCL21 |
| الوصف: | Chemokines orchestrate the organization of leucocyte recruitment during inflammation and homeostasis. Despite growing knowledge of chemokine receptors, some orphan chemokine receptors are still not characterized. The gene CCRL2 encodes such a receptor that exists in two splice variants, CRAM-A and CRAM-B. Here, we report that CRAM is expressed by human peripheral blood and bone marrow B cells, and by different B-cell lines dependent on the B-cell maturation stage. Intriguingly, CRAM surface expression on the pre-B-cell lines Nalm6 and G2 is specifically upregulated in response to the inflammatory chemokine CCL5 (RANTES), a chemokine that is well known to play an important role in modulating immune responses. Although Nalm6 cells do not express any of the known CCL5 binding receptors, extracellular signal-regulated kinases 1 and 2 (ERK1/2) are phosphorylated upon CCL5 stimulation, suggesting a direct effect of CCL5 through the CRAM receptor. However, no calcium mobilization or migratory responses upon CCL5 stimulation are induced in B-cell lines or in transfected cells. Also, ERK1/2 phosphorylation cannot be inhibited by pertussis toxin, suggesting that CRAM does not couple to Gi proteins. Our results describe the expression of a novel, non-classical chemokine receptor on B cells that is potentially involved in immunomodulatory functions together with CCL5. |
| تدمد: | 1365-2567 0019-2805 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a05bbabce97a85a2719796df525d0cc https://doi.org/10.1111/j.1365-2567.2008.02836.x |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....0a05bbabce97a85a2719796df525d0cc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652567 00192805 |
|---|